Figure 4
Tumor growth inhibition and decrease in leukemoid reaction in BALB/C mice by P2Et fraction. BALB/c mice implanted SC with 1 × 104 4T1 cells for 5 days and randomly divided into three groups. Group 1 was treated with PBS (vehicle), group 2 was treated with 18.7 mg/kg of P2Et fraction, and group 3 was treated with 9.3 mg/kg of P2Et fraction. Leukocyte count was determined weekly using a hemocytometer (ABX Micros 60). After 31 days, all animals were sacrificed. (a) Tumor volume. The graph represents the mean of each group with 7–8 animals per group. (b) Tumor weight. The graph represents the total animals by each group. For these experiments we used 7–8 animals per group and each experiment was performed independently two times. (c) Leukocyte count. The graph represents the total number animals used per group. For these experiments we used 7–8 animals per group and each experiment was performed independently two times.