Anti-protozoal activity of aporphine and protoberberine alkaloids from Annickia kummeriae (Engl. & Diels) Setten & Maas (Annonaceae)

Table 3 Anti-protozoal activity (IC ₅₀ ) and cytotoxicity (CC ₅₀ ) data of alkaloids from Annickia kummeriae

	P. falciparum K1		T.b. rhodesiense		L. donovani		L-6 cells
Compound	IC₅₀±S.E (μg/ml)	SI	IC₅₀±S.E (μg/ml)	SI	IC₅₀±S.E (μg/ml)	SI	CC₅₀± S.E (μg/ml)
Lysicamine (1)	2.4 ± 0.642	1.5	3.7 ± 0.001	2.3	2.7 ± 0.001	1.7	1.6 ± 0.01
Trivalvone (2)	1.6 ± 0.232	28.3	14.3 ± 0.001	3.2	2.9 ± 0.001	15.6	45.3 ± 0.02
Palmatine (3)	0.080 ± 0.001	1,154	3.2 ± 0.004	28.1	7.8 ± 0.001	11.5	>90
Jatrorrhizine (4)	0.24 ± 0.002	375.0	4.2 ± 0.002	21.4	20.4 ± 0.03	4.4	>90
Jatrorrhizine (4)/columbamine (5)	0.14 ± 0.017	358.6	4.0 ± 0.001	12.6	13.1 ± 0.02	3.8	50.2 ± 0.08
Palmatine (3)/tetrahydro- palmatine (6)	0.098 ± 0.002	629.6	4.3 ± 0.005	14.4	7.0 ± 0.06	8.81	61.7 ± 0.01

P. falciparum – K1 strain, T. b. rhodesiense – STIB 900 strain, L. donovani - MHOM-ET-67/L82, L-6 - rat skeletal myoblast cells, IC₅₀ – inhibitory concentration for 50% of tested parasites, CC₅₀ – cytotoxic concentration for 50% of tested cells, chloroquine IC₅₀ 0.063 ± 0.03, artemisinin IC₅₀ 0.002 ± 0.0001, melarsoprol IC₅₀ 0.002 ± 0.0001, miltefosine IC₅₀ 0.11 ± 0.001, podophyllotoxin IC₅₀ 0.009 ± 0.0003.

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