Anti-protozoal activity of aporphine and protoberberine alkaloids from Annickia kummeriae (Engl. & Diels) Setten & Maas (Annonaceae)

Table 2 Anti-plasmodial activity (IC ₅₀ ) and cytotoxicity (CC ₅₀ ) data of sub-fractions of fraction 2 of Annickia kummeriae methanolic leaf extract

Fraction	Weight (mg)	P. falciparum K1 IC₅₀(μg/ml)	Cytotoxicity CC₅₀(μg/ml)	SI	IC₅₀fr. IC₅₀CQ	IC₅₀fr. IC₅₀Art	CC₅₀fr. CC₅₀Pdx
AKLM 2	2,500	0.90 ± 0.11	21.0 ± 3.87	23.3	14	450	3,500
AKLM 2.1	15.3	5.0 ± 1.31	>90	18.0	78	2,500	>15,000
AKLM 2.2	26.9	1.09 ± 0.26	79.1 ± 7.60	72.6	17	545	13,183
AKLM 2.3	57.6	4.11 ± 0.29	53.1 ± 9.20	12.9	64	2,055	8,850
AKLM 2.4	98.7	1.16 ± 0.18	13.8 ± 1.80	11.9	18	580	2,300
AKLM 2.5	126.1	3.63 ± 0.04	24.6 ± 3.30	6.8	57	1,815	4,100
AKLM 2.6	115.7	1.23 ± 0.31	39.5 ± 2.20	32.1	19	615	6,583
AKLM 2.7	239.0	3.41 ± 0.48	85.4 ± 4.60	25.0	53	1,705	14,233
AKLM 2.8	478.3	5.0 ± 0.53	>90	18.0	78	2,500	>15,000
AKLM 2.9	351.8	2.40 ± 0.57	56.7 ± 8.00	23.6	38	1,200	9,450
AKLM 2.10	301.9	0.89 ± 0.20	35.3 ± 5.31	39.7	14	445	5,883
AKLM 2.11	672.8	0.64 ± 0.34	44.7 ± 4.45	69.8	10	320	7,450

HSCCC High speed counter current chromatography; AKLM Annickia kummeriae leaf methanol extract, P. falciparum K1 used for anti-plasmodial assays, rat myoblast L-6 cells used for cytotoxicity assays, CQ chloroquine (IC₅₀ 0.063 ± 0.034 μg/ml), Art - artemisinin (IC₅₀ 0.002 ± 0.00001 μg/ml), Pdx - podophyllotoxin (IC₅₀ 0.009 ± 0.003 μg/ml).

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