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Figure 3 | BMC Complementary and Alternative Medicine

Figure 3

From: Induction of selective cytotoxicity and apoptosis in human T4-lymphoblastoid cell line (CEMss) by boesenbergin a isolated from boesenbergia rotunda rhizomes involves mitochondrial pathway, activation of caspase 3 and G2/M phase cell cycle arrest

Figure 3

Confocal micrograph of acridine orange and propidium iodide double-stained CEMss cells. Cells were treated at IC50 of Boesenbergin A at time-dependent manner. (A) Untreated cells showed normal structure without prominent apoptosis and necrosis. (B) Early apoptosis features were seen after 24 h representing intercalated acridine orange (bright green) amongst the fragmented DNA (arrows). (C) Blebbing and nuclear margination were noticeable after 48 h treatment (arrows). (D) Late apoptosis was seen after 72 h post-treatment whereby a positive staining of orange color represents hallmark of late apoptosis (arrows). Figure 3B Histogram representing qualitative analysis of confocal micrograph consisting of acridine orange and propidium iodide double-stained treated and untreated CEMss cells. CEMss cell death via apoptosis increased significantly (p < 0.05) in a time-dependent manner. However, no significant (p > 0.05) difference was observed in the cell count of necrosis. (EA: early apoptosis; LA: late apoptosis; SN: secondary necrosis). ‘*’ indicates significant differences from the control (p < 0.05).

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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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