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Table 3 Method validation for the quantitative determination of six phenolic compounds using RP-HPLC

From: Phytochemical profiles, antioxidant and antimicrobial activities of three Potentilla species

Peak no.

Compounds

Regression equation

Precision experiment

Repeatability

Recovery experiment

Area of peak

RSD (%)

Area of peak

RSD (%)

Average recovery rate (%)

RSD (%)

4

(+)-catechin

y = 2004.7x - 25.13(R2 = 0.9973)

83.12 ± 0.61

0.73

12.82 ± 0.22

1.74

101.54 ± 3.08

1.03

7

Caffeic acid

y = 146577x + 12.70(R2 = 0.9998)

323.05 ± 1.83

1.32

285.04 ± 2.94

2.94

102.66 ± 2.96

2.88

10

Ferulic acid

y = 141714x - 14.70(R2 = 0.9998)

416.81 ± 0.92

1.88

32.85 ± 0.87

1.32

105.95 ± 2.44

2.3

13

Hyperoside

y = 66172x + 37.96(R2 = 0.9986)

960.32 ± 5.22

2.11

5801.02 ± 17.51

2.66

105.72 ± 1.56

1.48

14

Rutin

y = 43663x + 30.06(R2 = 0.9964)

89.79 ± 0.65

0.76

239.68 ± 7.85

0.65

96.21 ± 0.34

0.36

16

Ellagic acid

y = 62654x - 82.44(R2 = 0.9999)

101.25 ± 1.43

0.93

181.42 ± 2.40

3.28

102.60 ± 1.20

1.17

  1. Values were expressed in mean ± SD (N = 6).