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Table 3 Method validation for the quantitative determination of six phenolic compounds using RP-HPLC

From: Phytochemical profiles, antioxidant and antimicrobial activities of three Potentilla species

Peak no. Compounds Regression equation Precision experiment Repeatability Recovery experiment
Area of peak RSD (%) Area of peak RSD (%) Average recovery rate (%) RSD (%)
4 (+)-catechin y = 2004.7x - 25.13(R2 = 0.9973) 83.12 ± 0.61 0.73 12.82 ± 0.22 1.74 101.54 ± 3.08 1.03
7 Caffeic acid y = 146577x + 12.70(R2 = 0.9998) 323.05 ± 1.83 1.32 285.04 ± 2.94 2.94 102.66 ± 2.96 2.88
10 Ferulic acid y = 141714x - 14.70(R2 = 0.9998) 416.81 ± 0.92 1.88 32.85 ± 0.87 1.32 105.95 ± 2.44 2.3
13 Hyperoside y = 66172x + 37.96(R2 = 0.9986) 960.32 ± 5.22 2.11 5801.02 ± 17.51 2.66 105.72 ± 1.56 1.48
14 Rutin y = 43663x + 30.06(R2 = 0.9964) 89.79 ± 0.65 0.76 239.68 ± 7.85 0.65 96.21 ± 0.34 0.36
16 Ellagic acid y = 62654x - 82.44(R2 = 0.9999) 101.25 ± 1.43 0.93 181.42 ± 2.40 3.28 102.60 ± 1.20 1.17
  1. Values were expressed in mean ± SD (N = 6).