Antimicrobial activity and cytotoxicity of the ethanol extract, fractions and eight compounds isolated from Eriosema robustum (Fabaceae)

Table 2 Total activity in ml/g of crude extract and fractions (A and B) from E. robustum calculated by dividing mass in g of extract or fractions with their MIC values[20]

Samples	Fungi									Bacteria
	C.a		C.A		A.f		C.n		Average	S.a	P.a	E.f	E.c	Average
	16 h	24 h	16 h	24 h	16 h	24 h	24 h	36 h
Extract and Fractions
Crude extract	4000	4000	4000	4000	31250	7937	7937	7937	8883	62500	16129	62500	62500	50907
Fraction A	1429	1429	5625	2813	2813	1429	720	720	2123	45000	2903	22500	22500	23226
Fraction B	560	560	2188	1111	8750	1111	8750	1111	3018	4375	2258	8750	8750	6033
Compounds
Robusflavone A (1)	4	4	13.4	13.4	13.4	8	4	4	8	4	16	4	4	7
Robusflavone B (2)	8	8	13.4	13.4	13.4	8	13.4	13.4	11	8	16	16	8	12
Orostachyscerebroside A (3)	4	4	7.7	4	7.7	4	4	4	4.5	8	16	8	8	9.1
Stigmasterol (4)	8	8	8	8	13.4	13.4	8	8	9	8	16	4	4	8
1-O-Heptatriacontanoyl glycerol (5)	4	4	13.4	13.4	8	8	4	4	7	8	16	8	8	10
Eicosanoic acid (6)	4	4	8	8	8	8	< 8	< 8	7	16	16	8	8	12
3-O-β-D-Glucopyranoside of sitosterol (7)	8	8	8	8	13.4	8	13.4	8	9	8	16	16	8	12
6-Prenylpinocembrin (8)	8	8	13.4	13.4	8	4	< 4	< 4	8	32	16	16	16	20

C.a: Candida albicans (isolate), C.A: Candida albicans (ATCC), C.n: Cryptococcus neoformans, A.f: Aspergillus fumigatus, E.c: Escherichia coli, E.f: Enterococcus faecalis, S.a: Staphylococcus aureus, P.a: Pseudomonas aeruginosa.
The inverse of MIC in ml/mg was calculated for compounds (1 – 8).

ISSN: 2662-7671