Antimicrobial activity and cytotoxicity of the ethanol extract, fractions and eight compounds isolated from Eriosema robustum (Fabaceae)

Table 1 Minimum inhibitory concentration (MIC) of the extract, fractions and compounds (1–8) from E. robustum against fungi (C.a, C.A, A.f, C.n) and bacteria (S.a, P.a, E.f, E.c)

Samples	Fungi (MIC values)									Bacteria (MIC values)
	C.a		C.A		A.f		C.n^a		Average	S.a	P.a	E.f	E.c	Average
	16 h	24 h	16 h	24 h	16 h	24 h	24 h	36 h
Extract and Fractions (mg/ml)
Crude extract	1.25	1.25	1.25	1.25	0.16	0.63	0.63	0.63	0.88	0.08	0.31	0.08	0.08	0.14
Fraction A	0.63	0.63	0.16	0.32	0.32	0.63	1.25	1.25	0.65	0.02	0.31	0.04	0.04	0.10
Fraction B	1.25	1.25	0.32	0.63	0.08	0.63	0.08	0.63	0.61	0.16	0.31	0.08	0.08	0.16
Compounds (μg/ml)
Robusflavone A (1)	250	250	65	65	65	125	250	250	165	250	63	250	250	203.3
Robusflavone B (2)	125	125	65	65	65	125	65	65	88	125	63	63	125	94
Orostachyscerebroside A (3)	250	250	130	250	130	250	250	250	220	125	63	125	125	109.5
Stigmasterol (4)	125	125	125	125	65	65	125	125	110	125	63	250	250	172
1-O-Heptatriacontanoyl glycerol (5)	250	250	65	65	125	125	250	250	173	125	63	125	125	109.5
Eicosanoic acid (6)	250	250	125	125	125	125	> 250	> 250	188	63	63	125	125	94
3-O-β-D-Glucopyranoside of sitosterol (7)	125	125	125	125	65	125	65	125	110	125	63	63	125	94
6-Prenylpinocembrin (8)	125	125	65	65	125	250	> 250	> 250	157	31.3	63	63	63	55.1
Controls (μg/ml)
Amp B	30	30	10	30	65	125	> 250	> 250	99	-	-	-	-	-
Gen	-	-	-	-	-	-	-	-	-	< 3.91	< 3.91	< 3.91	< 3.91	< 3.91
DMSO^b	na	na	na	na	na	na	na	na		na	na	na	na

C.a: Candida albicans (isolate), C.A: Candida albicans (ATCC), C.n: Cryptococcus neoformans, A.f: Aspergillus fumigatus, E.c: Escherichia coli, E.f: Enterococcus faecalis, S.a: Staphylococcus aureus, P.a: Pseudomonas aeruginosa, Amp B: Amphotericin B, Gen: Gentamicin, ^aWith this microorganism little reaction was observed after 16 h and values were measured after 24 and 36 h, ^bThe final concentration of DMSO used was less than 10%, na: not active.
The results are the means of three replicates for fungi and two replicates for bacteria and the standard deviation was zero.

ISSN: 2662-7671