Figure 4

EA microdialysates enhanced neurosphere growth and p-ERK level of NSCs. (A) % cell viability of stem cells in the Model and EA medium; (B) The formation of big-spheres (diameter ≥ 100 μm) number/100 μl in different mediums; (C) The p-ERK + stem cells in an average visual field under the 10× objective lens after 100 μl NSC suspension was fixed to a 96-well plate and stained. The values are presented as the mean ± SE, n = 3, # P <0.05 (EA vs. Model).