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Table 2 Gravity column chromatographic sub-fractions (SF) of A. loheri CF1 run in silica gel-60 and various gradients of increasing amount of ethyl acetate

From: A phenolic ester from Aglaia loheri leaves reveals cytotoxicity towards sensitive and multidrug-resistant cancer cells

 

Gradient

 

Sub-fractions (SF)

(Percent ratio of eluent: hexane to ethyl acetate)

IC50

  

(μg/mL)

1

85:15

NLI

2

83:17

42.06 ± 0.67

3

81:19

35.17 ± 4.63

4

78:22

31.61 ± 6.52

5

75:25

40.55 ± 7.24

6

71:29

NLI

7

67:33

NLI

8

63:37

NLI

9

59:41

3.96 ± 0.07

10

55:45

4.98 ± 0.50

11

50:50

11.92 ± 1.29

Doxorubicin

 

4.31 ± 0.06

  1. NLI: No Logarithmic Interpolation indicating absence of toxicity.
  2. Bold Texts: Most cytotoxic sub-fraction of CF1 (Table 1).