Figure 4

Determination of mitochondrial membrane potential (ΔΨm) in multidrug-resistant CEM/ADR5000 cells after 24 and 48 h exposure to Maldi 531.2[M + H] ⁺ . Data are means ± SEM of the three replicates of two independent experiments; P < 0.05. Untreated cells served as negative control and 10 μg/mL doxorubicin (doxo) and 0.05 μM carbonyl cyanide 3-chlorophenyl hydrazone (CCCP)-treated cells as positive controls. Significant differences between are indicated by letters. After 24 hr treatment, mitochondrial membrane potential (ΔΨm) of untreated cells (UT-S), indicated by subset “a” is significantly different from the CCCP and Maldi 531.2[M + H] + treated cells as indicated by subset “b”. Values for CCCP and Maldi 531.2[M + H] + treated cells don’t show significant differences from each other as belong to the same subset “b”. Mitochondrial membrane potential (ΔΨm) of untreated cells (UT-S), indicated by subset “x” is significantly different from the CCCP and Maldi 531.2[M + H] + treated cells as indicated by subset “y” and “z” for 48 hrs. Values of same subset at non-significant from each other.