Polygonum cuspidatum inhibits pancreatic lipase activity and adipogenesis via attenuation of lipid accumulation

Table 1 Sequences of primers and PCR conditions for semi-quantitative RT-PCR

Gene	Sequence (5′-3′)	Annealing temperature (°C)	Size (bp)	Accession No.
ADRP		55	352	NM_00748
Sense	CTT GTG TCC TCC GCT TAT GTC AGT
Anti-sense	CTG CTC CTT TGG TCT TAT CCA CCA
Perilipin		55	508	NM_175640
Sense	CTT TCT CGA CAC ACC ATG GAA ACC
Anti-sense	CCA CGT TAT CCG TAA CAC CCT TCA
PPAR-γ		55	220	NM_011146
Sense	CCA GAG CAT GGT GGG TTC GCT G
Anti-sense	GAG CTG ACC CAA TGG TTG CTG
C/EBP-α		55	238	NM_007678
Sense	AGG TGG TGG AGT TGA CCA GT
Anti-sense	CAG CCT AGA GAT CCA GCG AC

ISSN: 2662-7671