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Table 3 Identified proteins of A549 downregulated (-) in the presence of methanolic Phyllanthus extracts

From: Inhibition of Raf-MEK-ERK and Hypoxia pathways by Phyllanthus prevents metastasis in human lung (A549) cancer cell line

Spot ID

Possible proteins

Fold change

UNIPROT KB/SWISS-PROT Acc. Number

Database

MPN

MPU

MPW

MPA

1

Protein S100-A8

-0.47

-0.10

-0.48

-0.55

P05109

MASCOT;UNIPROT KB/SWISS-PROT;COGS

2

Transmembrane protein 35

-0.82

-0.78

-1.00

-0.50

Q53FP2

MASCOT;UNIPROT KB/SWISS-PROT;COGS

3

Adenylate kinase isoenzyme 6

-0.32

-0.62

-0.81

-0.73

Q9Y3D8

MASCOT;UNIPROT KB/SWISS-PROT;COGS

4

Peroxiredoxin-1

-0.78

-1.00

-1.00

-1.00

Q06830

MASCOT;UNIPROT KB/SWISS-PROT;COGS

5

Prolactin-releasing peptide precursor

-0.59

-0.29

-0.23

-0.39

P81277

MASCOT;UNIPROT KB/SWISS-PROT;COGS

6

Putative protein SSX6

-0.46

-0.96

-1.00

-0.75

Q7RTT6

MASCOT;UNIPROT KB/SWISS-PROT;COGS

7

Glutathione synthetase

-0.38

-0.79

-1.00

-0.51

P48637

MASCOT;UNIPROT KB/SWISS-PROT;COGS

8

Nucleoside diphosphate-linked moiety X motif 16

-0.61

-0.63

-0.98

-0.94

Q3MHX9

MASCOT;UNIPROT KB/SWISS-PROT;COGS

9

E3 ubiquitin-protein ligase ZNRF1

N/A

N/A

-0.47

-0.36

Q8ND25

MASCOT;UNIPROT KB/SWISS-PROT;COGS

10

DNA-directed RNA polymerase II 16 kDa polypeptide

-0.46

-0.59

-0.43

-0.93

Q9VEA5

MASCOT;UNIPROT KB/SWISS-PROT;COGS

11

39S ribosomal protein L40, mitochondrial precursor

-1.00

-1.00

-1.00

-1.00

Q9NQ50

MASCOT;UNIPROT KB/SWISS-PROT;COGS

12

Zinc finger protein 174

-1.00

-1.00

-1.00

-1.00

Q15697

MASCOT;UNIPROT KB/SWISS-PROT;COGS

13

Probable G-protein coupled receptor 179 precursor

-0.21

-0.15

-1.00

N/A

Q6PRD1

MASCOT;UNIPROT KB/SWISS-PROT;COGS

14

Histatin-1 precursor

-0.24

-0.66

-0.78

-0.27

P15515

MASCOT;UNIPROT KB/SWISS-PROT;COGS

15

U6 snRNA-associated Sm-like protein LSm5

-0.33

-0.57

-0.19

-0.54

Q9Y4Y9

MASCOT;UNIPROT KB/SWISS-PROT;COGS

16

Bcl-2-like protein 11

-0.25

-1.00

-1.00

-0.70

O43521

MASCOT;UNIPROT KB/SWISS-PROT;COGS

17

Contactin-2 precursor

-0.29

-0.60

-0.42

-0.39

Q02246

MASCOT;UNIPROT KB/SWISS-PROT;COGS

18

Bis(5′-adenosyl)-triphosphatase

-1.00

-1.00

N/A

-1.00

P49789

MASCOT;UNIPROT KB/SWISS-PROT;COGS

19

Trypsin-1 precursor

-1.00

-0.98

-1.00

-1.00

P07477

MASCOT;UNIPROT KB/SWISS-PROT;COGS

20

Proliferating cell nuclear antigen

-1.00

-1.00

-1.00

-1.00

P12004

MASCOT;UNIPROT KB/SWISS-PROT;COGS

21

Endoplasmin precursor

N/A

-1.00

-1.00

-0.37

P14625

MASCOT;UNIPROT KB/SWISS-PROT;COGS

22

Eukaryotic translation initiation factor 3 subunit 12

-1.00

-1.00

-1.00

-1.00

Q9UBQ5

MASCOT;UNIPROT KB/SWISS-PROT;COGS

23

Serine/threonine-protein kinase 6

-1.00

-0.96

-0.19

-1.00

O14965

MASCOT;UNIPROT KB/SWISS-PROT;COGS

24

Phenylalanyl-tRNA synthetase beta chain

-1.00

-1.00

-1.00

-1.00

Q9NSD9

MASCOT;UNIPROT KB/SWISS-PROT;COGS

25

Neutrophil defensin 1 precursor

-0.78

-0.49

-0.20

-0.88

P59665

MASCOT;UNIPROT KB/SWISS-PROT;COGS

26

Proto-oncogene protein Wnt-3 precursor

-0.58

N/A

-0.34

-0.55

P56703

MASCOT;UNIPROT KB/SWISS-PROT;COGS

27

Putative Ras-related protein Rab-42

-1.00

N/A

-0.97

-0.80

Q8N4Z0

MASCOT;UNIPROT KB/SWISS-PROT;COGS

28

UPF0404 protein C11orf59

-0.19

-0.36

N/A

-0.36

Q6IAA8

MASCOT;UNIPROT KB/SWISS-PROT;COGS

29

Neutrophil defensin 1 precursor

-0.31

-0.25

-1.00

-0.87

P59665

MASCOT;UNIPROT KB/SWISS-PROT;COGS

30

Agouti-signaling protein precursor

-0.33

-0.67

-0.67

N/A

P42127

MASCOT;UNIPROT KB/SWISS-PROT;COGS

31

DNA-directed RNA polymerase II 16 kDa polypeptide

-0.42

-0.48

-0.48

-0.45

Q9VEA5

MASCOT;UNIPROT KB/SWISS-PROT;COGS

32

Beta-defensin 107A precursor

-0.21

N/A

-0.62

-0.61

Q8IZN7

MASCOT;UNIPROT KB/SWISS-PROT;COGS

33

Transcription elongation factor B polypeptide 1

-0.58

-0.59

-0.56

-0.79

Q15369

MASCOT;UNIPROT KB/SWISS-PROT;COGS

34

Metallothionein-1M

-0.55

-0.52

-0.48

-0.48

Q8N339

MASCOT;UNIPROT KB/SWISS-PROT;COGS

35

GTPase HRas precursor

-0.60

-0.66

-0.59

-0.61

P01112

MASCOT;UNIPROT KB/SWISS-PROT;COGS

36

Neuromedin-B precursor

-0.24

-0.32

-0.23

-0.34

P08949

MASCOT;UNIPROT KB/SWISS-PROT;COGS

37

Protein-tyrosine sulfotransferase 2

-0.36

N/A

N/A

-0.33

O60704

MASCOT;UNIPROT KB/SWISS-PROT;COGS

38

Apolipoprotein A-II precursor

-0.23

-0.23

-0.13

-0.37

P02652

MASCOT;UNIPROT KB/SWISS-PROT;COGS

39

ADP-ribosylation factor-like protein 6

-0.58

-0.52

-1.00

-0.29

Q9H0F7

MASCOT;UNIPROT KB/SWISS-PROT;COGS

40

Alpha-enolase

-0.27

-0.51

N/A

-0.56

P06733

MASCOT;UNIPROT KB/SWISS-PROT;COGS

41

Serine/threonine-protein phosphatase PP1-beta catalytic subunit

-1.00

-1.00

-0.44

-0.71

P62140

MASCOT;UNIPROT KB/SWISS-PROT;COGS

42

N-acylneuraminate cytidylyltransferase

-1.00

-1.00

-1.00

-1.00

Q8NFW8

MASCOT;UNIPROT KB/SWISS-PROT;COGS

43

Inosine triphosphate pyrophosphatase

-1.00

-1.00

-1.00

-1.00

Q9BY32

MASCOT;UNIPROT KB/SWISS-PROT;COGS

44

Alkyldihydroxyacetonephosphate synthase

-1.00

-0.55

-1.00

-1.00

O00116

MASCOT;UNIPROT KB/SWISS-PROT;COGS

45

Microsomal signal peptidase 18 kDa subunit

-1.00

-0.57

-1.00

-1.00

P67812

MASCOT;UNIPROT KB/SWISS-PROT;COGS

46

Tumor suppressor candidate 2

-0.89

-0.75

-0.67

-0.21

O75896

MASCOT;UNIPROT KB/SWISS-PROT;COGS

47

Metallothionein-2

-0.71

-0.17

-0.14

-0.53

P02795

MASCOT;UNIPROT KB/SWISS-PROT;COGS

48

Protein FAM3B precursor

-0.57

-0.48

-0.39

-0.48

P58499

MASCOT;UNIPROT KB/SWISS-PROT;COGS

49

Metallothionein-1H

-0.52

-0.48

N/A

-0.29

P80294

MASCOT;UNIPROT KB/SWISS-PROT;COGS

50

Metallothionein-2

-0.34

-0.59

-0.49

-1.00

P02795

MASCOT;UNIPROT KB/SWISS-PROT;COGS

51

Protein BEX5

-0.48

-0.33

-0.30

-0.48

Q5H9J7

MASCOT;UNIPROT KB/SWISS-PROT;COGS

52

DNA-directed RNA polymerase II 16 kDa polypeptide

-1.00

-1.00

-1.00

-1.00

Q9VEA5

MASCOT;UNIPROT KB/SWISS-PROT;COGS

53

Cytochrome c oxidase polypeptide VIa-liver, mitochondrial precursor

-0.93

-0.24

-1.00

-0.14

P12074

MASCOT;UNIPROT KB/SWISS-PROT;COGS

54

Putative protein 15E1.2

N/A

-0.94

-1.00

-0.30

-

MASCOT;COGS

55

Voltage-dependent anion-selective channel protein 1

-0.15

-0.28

-0.35

-0.27

P21796

MASCOT;UNIPROT KB/SWISS-PROT;COGS

56

Voltage-dependent anion-selective channel protein 1

-0.47

-0.13

-1.32

N/A

P21796

MASCOT;UNIPROT KB/SWISS-PROT;COGS

57

Serine/threonine-protein phosphatase PP1-alpha catalytic subunit

-0.64

-0.76

-0.43

-0.32

P62136

MASCOT;UNIPROT KB/SWISS-PROT;COGS

58

Acetyl-CoA acetyltransferase, cytosolic

-0.95

-1.00

-0.17

-1.00

Q9BWD1

MASCOT;UNIPROT KB/SWISS-PROT;COGS

59

Glutathione synthetase

-0.50

-0.45

-0.38

-0.61

P48637

MASCOT;UNIPROT KB/SWISS-PROT;COGS

60

Actin-related protein 10

-0.94

-0.70

-0.13

-0.73

Q9NZ32

MASCOT;UNIPROT KB/SWISS-PROT;COGS

61

Enteropeptidase precursor

-0.63

-0.19

-1.00

-0.70

P98073

MASCOT;UNIPROT KB/SWISS-PROT;COGS

62

Metallothionein-1L

-0.86

-0.79

-0.96

-0.44

Q93083

MASCOT;UNIPROT KB/SWISS-PROT;COGS

63

Guanine nucleotide-binding protein G(I)/G(S)/G(O) gamma-5-like subunit

-1.00

-1.00

-1.00

-1.00

P63218

MASCOT;UNIPROT KB/SWISS-PROT;COGS

64

Small inducible cytokine B14 precursor

-1.00

-1.00

-1.00

-1.00

O95715

MASCOT;UNIPROT KB/SWISS-PROT;COGS

  1. The negative (-) symbol signified suppression of the proteins and a value of 1.00 indicates the complete absence of this protein in the methanolic Phyllanthus-treated sample as compared to the untreated-control sample and the degree of suppression reduces as the value increases. MPN – Methanolic P. niruri, MPU – Methanolic P. urinaria, MPW – Methanolic P. watsonii, MPA – Methanolic P. amarus, N/A – Not Affected.