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Table 3 Identified proteins of A549 downregulated (-) in the presence of methanolic Phyllanthus extracts

From: Inhibition of Raf-MEK-ERK and Hypoxia pathways by Phyllanthus prevents metastasis in human lung (A549) cancer cell line

Spot ID Possible proteins Fold change UNIPROT KB/SWISS-PROT Acc. Number Database
MPN MPU MPW MPA
1 Protein S100-A8 -0.47 -0.10 -0.48 -0.55 P05109 MASCOT;UNIPROT KB/SWISS-PROT;COGS
2 Transmembrane protein 35 -0.82 -0.78 -1.00 -0.50 Q53FP2 MASCOT;UNIPROT KB/SWISS-PROT;COGS
3 Adenylate kinase isoenzyme 6 -0.32 -0.62 -0.81 -0.73 Q9Y3D8 MASCOT;UNIPROT KB/SWISS-PROT;COGS
4 Peroxiredoxin-1 -0.78 -1.00 -1.00 -1.00 Q06830 MASCOT;UNIPROT KB/SWISS-PROT;COGS
5 Prolactin-releasing peptide precursor -0.59 -0.29 -0.23 -0.39 P81277 MASCOT;UNIPROT KB/SWISS-PROT;COGS
6 Putative protein SSX6 -0.46 -0.96 -1.00 -0.75 Q7RTT6 MASCOT;UNIPROT KB/SWISS-PROT;COGS
7 Glutathione synthetase -0.38 -0.79 -1.00 -0.51 P48637 MASCOT;UNIPROT KB/SWISS-PROT;COGS
8 Nucleoside diphosphate-linked moiety X motif 16 -0.61 -0.63 -0.98 -0.94 Q3MHX9 MASCOT;UNIPROT KB/SWISS-PROT;COGS
9 E3 ubiquitin-protein ligase ZNRF1 N/A N/A -0.47 -0.36 Q8ND25 MASCOT;UNIPROT KB/SWISS-PROT;COGS
10 DNA-directed RNA polymerase II 16 kDa polypeptide -0.46 -0.59 -0.43 -0.93 Q9VEA5 MASCOT;UNIPROT KB/SWISS-PROT;COGS
11 39S ribosomal protein L40, mitochondrial precursor -1.00 -1.00 -1.00 -1.00 Q9NQ50 MASCOT;UNIPROT KB/SWISS-PROT;COGS
12 Zinc finger protein 174 -1.00 -1.00 -1.00 -1.00 Q15697 MASCOT;UNIPROT KB/SWISS-PROT;COGS
13 Probable G-protein coupled receptor 179 precursor -0.21 -0.15 -1.00 N/A Q6PRD1 MASCOT;UNIPROT KB/SWISS-PROT;COGS
14 Histatin-1 precursor -0.24 -0.66 -0.78 -0.27 P15515 MASCOT;UNIPROT KB/SWISS-PROT;COGS
15 U6 snRNA-associated Sm-like protein LSm5 -0.33 -0.57 -0.19 -0.54 Q9Y4Y9 MASCOT;UNIPROT KB/SWISS-PROT;COGS
16 Bcl-2-like protein 11 -0.25 -1.00 -1.00 -0.70 O43521 MASCOT;UNIPROT KB/SWISS-PROT;COGS
17 Contactin-2 precursor -0.29 -0.60 -0.42 -0.39 Q02246 MASCOT;UNIPROT KB/SWISS-PROT;COGS
18 Bis(5′-adenosyl)-triphosphatase -1.00 -1.00 N/A -1.00 P49789 MASCOT;UNIPROT KB/SWISS-PROT;COGS
19 Trypsin-1 precursor -1.00 -0.98 -1.00 -1.00 P07477 MASCOT;UNIPROT KB/SWISS-PROT;COGS
20 Proliferating cell nuclear antigen -1.00 -1.00 -1.00 -1.00 P12004 MASCOT;UNIPROT KB/SWISS-PROT;COGS
21 Endoplasmin precursor N/A -1.00 -1.00 -0.37 P14625 MASCOT;UNIPROT KB/SWISS-PROT;COGS
22 Eukaryotic translation initiation factor 3 subunit 12 -1.00 -1.00 -1.00 -1.00 Q9UBQ5 MASCOT;UNIPROT KB/SWISS-PROT;COGS
23 Serine/threonine-protein kinase 6 -1.00 -0.96 -0.19 -1.00 O14965 MASCOT;UNIPROT KB/SWISS-PROT;COGS
24 Phenylalanyl-tRNA synthetase beta chain -1.00 -1.00 -1.00 -1.00 Q9NSD9 MASCOT;UNIPROT KB/SWISS-PROT;COGS
25 Neutrophil defensin 1 precursor -0.78 -0.49 -0.20 -0.88 P59665 MASCOT;UNIPROT KB/SWISS-PROT;COGS
26 Proto-oncogene protein Wnt-3 precursor -0.58 N/A -0.34 -0.55 P56703 MASCOT;UNIPROT KB/SWISS-PROT;COGS
27 Putative Ras-related protein Rab-42 -1.00 N/A -0.97 -0.80 Q8N4Z0 MASCOT;UNIPROT KB/SWISS-PROT;COGS
28 UPF0404 protein C11orf59 -0.19 -0.36 N/A -0.36 Q6IAA8 MASCOT;UNIPROT KB/SWISS-PROT;COGS
29 Neutrophil defensin 1 precursor -0.31 -0.25 -1.00 -0.87 P59665 MASCOT;UNIPROT KB/SWISS-PROT;COGS
30 Agouti-signaling protein precursor -0.33 -0.67 -0.67 N/A P42127 MASCOT;UNIPROT KB/SWISS-PROT;COGS
31 DNA-directed RNA polymerase II 16 kDa polypeptide -0.42 -0.48 -0.48 -0.45 Q9VEA5 MASCOT;UNIPROT KB/SWISS-PROT;COGS
32 Beta-defensin 107A precursor -0.21 N/A -0.62 -0.61 Q8IZN7 MASCOT;UNIPROT KB/SWISS-PROT;COGS
33 Transcription elongation factor B polypeptide 1 -0.58 -0.59 -0.56 -0.79 Q15369 MASCOT;UNIPROT KB/SWISS-PROT;COGS
34 Metallothionein-1M -0.55 -0.52 -0.48 -0.48 Q8N339 MASCOT;UNIPROT KB/SWISS-PROT;COGS
35 GTPase HRas precursor -0.60 -0.66 -0.59 -0.61 P01112 MASCOT;UNIPROT KB/SWISS-PROT;COGS
36 Neuromedin-B precursor -0.24 -0.32 -0.23 -0.34 P08949 MASCOT;UNIPROT KB/SWISS-PROT;COGS
37 Protein-tyrosine sulfotransferase 2 -0.36 N/A N/A -0.33 O60704 MASCOT;UNIPROT KB/SWISS-PROT;COGS
38 Apolipoprotein A-II precursor -0.23 -0.23 -0.13 -0.37 P02652 MASCOT;UNIPROT KB/SWISS-PROT;COGS
39 ADP-ribosylation factor-like protein 6 -0.58 -0.52 -1.00 -0.29 Q9H0F7 MASCOT;UNIPROT KB/SWISS-PROT;COGS
40 Alpha-enolase -0.27 -0.51 N/A -0.56 P06733 MASCOT;UNIPROT KB/SWISS-PROT;COGS
41 Serine/threonine-protein phosphatase PP1-beta catalytic subunit -1.00 -1.00 -0.44 -0.71 P62140 MASCOT;UNIPROT KB/SWISS-PROT;COGS
42 N-acylneuraminate cytidylyltransferase -1.00 -1.00 -1.00 -1.00 Q8NFW8 MASCOT;UNIPROT KB/SWISS-PROT;COGS
43 Inosine triphosphate pyrophosphatase -1.00 -1.00 -1.00 -1.00 Q9BY32 MASCOT;UNIPROT KB/SWISS-PROT;COGS
44 Alkyldihydroxyacetonephosphate synthase -1.00 -0.55 -1.00 -1.00 O00116 MASCOT;UNIPROT KB/SWISS-PROT;COGS
45 Microsomal signal peptidase 18 kDa subunit -1.00 -0.57 -1.00 -1.00 P67812 MASCOT;UNIPROT KB/SWISS-PROT;COGS
46 Tumor suppressor candidate 2 -0.89 -0.75 -0.67 -0.21 O75896 MASCOT;UNIPROT KB/SWISS-PROT;COGS
47 Metallothionein-2 -0.71 -0.17 -0.14 -0.53 P02795 MASCOT;UNIPROT KB/SWISS-PROT;COGS
48 Protein FAM3B precursor -0.57 -0.48 -0.39 -0.48 P58499 MASCOT;UNIPROT KB/SWISS-PROT;COGS
49 Metallothionein-1H -0.52 -0.48 N/A -0.29 P80294 MASCOT;UNIPROT KB/SWISS-PROT;COGS
50 Metallothionein-2 -0.34 -0.59 -0.49 -1.00 P02795 MASCOT;UNIPROT KB/SWISS-PROT;COGS
51 Protein BEX5 -0.48 -0.33 -0.30 -0.48 Q5H9J7 MASCOT;UNIPROT KB/SWISS-PROT;COGS
52 DNA-directed RNA polymerase II 16 kDa polypeptide -1.00 -1.00 -1.00 -1.00 Q9VEA5 MASCOT;UNIPROT KB/SWISS-PROT;COGS
53 Cytochrome c oxidase polypeptide VIa-liver, mitochondrial precursor -0.93 -0.24 -1.00 -0.14 P12074 MASCOT;UNIPROT KB/SWISS-PROT;COGS
54 Putative protein 15E1.2 N/A -0.94 -1.00 -0.30 - MASCOT;COGS
55 Voltage-dependent anion-selective channel protein 1 -0.15 -0.28 -0.35 -0.27 P21796 MASCOT;UNIPROT KB/SWISS-PROT;COGS
56 Voltage-dependent anion-selective channel protein 1 -0.47 -0.13 -1.32 N/A P21796 MASCOT;UNIPROT KB/SWISS-PROT;COGS
57 Serine/threonine-protein phosphatase PP1-alpha catalytic subunit -0.64 -0.76 -0.43 -0.32 P62136 MASCOT;UNIPROT KB/SWISS-PROT;COGS
58 Acetyl-CoA acetyltransferase, cytosolic -0.95 -1.00 -0.17 -1.00 Q9BWD1 MASCOT;UNIPROT KB/SWISS-PROT;COGS
59 Glutathione synthetase -0.50 -0.45 -0.38 -0.61 P48637 MASCOT;UNIPROT KB/SWISS-PROT;COGS
60 Actin-related protein 10 -0.94 -0.70 -0.13 -0.73 Q9NZ32 MASCOT;UNIPROT KB/SWISS-PROT;COGS
61 Enteropeptidase precursor -0.63 -0.19 -1.00 -0.70 P98073 MASCOT;UNIPROT KB/SWISS-PROT;COGS
62 Metallothionein-1L -0.86 -0.79 -0.96 -0.44 Q93083 MASCOT;UNIPROT KB/SWISS-PROT;COGS
63 Guanine nucleotide-binding protein G(I)/G(S)/G(O) gamma-5-like subunit -1.00 -1.00 -1.00 -1.00 P63218 MASCOT;UNIPROT KB/SWISS-PROT;COGS
64 Small inducible cytokine B14 precursor -1.00 -1.00 -1.00 -1.00 O95715 MASCOT;UNIPROT KB/SWISS-PROT;COGS
  1. The negative (-) symbol signified suppression of the proteins and a value of 1.00 indicates the complete absence of this protein in the methanolic Phyllanthus-treated sample as compared to the untreated-control sample and the degree of suppression reduces as the value increases. MPN – Methanolic P. niruri, MPU – Methanolic P. urinaria, MPW – Methanolic P. watsonii, MPA – Methanolic P. amarus, N/A – Not Affected.