Skip to main content

Table 2 Identified proteins of A549 downregulated (-) in the presence of aqueous Phyllanthus extracts

From: Inhibition of Raf-MEK-ERK and Hypoxia pathways by Phyllanthus prevents metastasis in human lung (A549) cancer cell line

Spot ID

Possible proteins

Fold change

UNIPROT KB/SWISS-PROT Acc. Number

Database

APN

APU

APW

APA

1

Actin, cytoplasmic 1

-0.81

-0.88

-0.95

-0.84

P60709

MASCOT;UNIPROT KB/SWISS-PROT;COGS

2

Nicotinamide N-methyltransferase

-0.50

N/A

-0.28

-0.10

P40261

MASCOT;UNIPROT KB/SWISS-PROT;COGS

3

Alpha-Enolase

-0.62

-0.57

-0.48

-0.58

P06733

MASCOT;UNIPROT KB/SWISS-PROT;COGS

4

Proteasome subunit beta type 3

-1.00

-0.20

N/A

-1.00

P49720

MASCOT;UNIPROT KB/SWISS-PROT;COGS

5

Retinal dehydrogenase 1

-0.23

N/A

-0.13

N/A

P00352

MASCOT;UNIPROT KB/SWISS-PROT;COGS

6

Galectin-1

-1.00

-0.13

-0.57

-0.38

P09382

MASCOT;UNIPROT KB/SWISS-PROT;COGS

7

Heat-shock protein beta-1

-0.12

-0.22

-0.32

-0.10

P04792

MASCOT;UNIPROT KB/SWISS-PROT;COGS

8

Glyceraldehyde-3-phosphate dehydrogenase

-0.30

-0.93

-0.99

-0.91

P04406

MASCOT;UNIPROT KB/SWISS-PROT;COGS

9

Eukaryotic translation initiation factor 5A-1

-0.32

-0.39

-0.27

-0.80

P63241

MASCOT;UNIPROT KB/SWISS-PROT;COGS

10

60 kDa heat shock protein, mitochondrial precursor

-0.46

-0.82

-0.90

-0.75

P10809

MASCOT;UNIPROT KB/SWISS-PROT;COGS

11

Glutathione transferase omega-1

N/A

-0.45

N/A

-0.22

P78417

MASCOT;UNIPROT KB/SWISS-PROT;COGS

12

Proteasome activator complex subunit 1

-0.19

-0.18

N/A

-1.00

Q06323

MASCOT;UNIPROT KB/SWISS-PROT;COGS

13

Annexin A4

-0.29

-0.29

-0.21

-0.65

P09525

MASCOT;UNIPROT KB/SWISS-PROT;COGS

14

Vimentin

-0.91

-0.82

-0.24

-0.79

P08670

MASCOT;UNIPROT KB/SWISS-PROT;COGS

15

Peptidyl-prolyl cis-trans isomerase A

-0.13

-0.36

-1.00

-0.42

P62937

MASCOT;UNIPROT KB/SWISS-PROT;COGS

16

Peptidyl-prolyl cis-trans isomerase A

-1.00

-1.00

-0.32

N/A

P62937

MASCOT;UNIPROT KB/SWISS-PROT;COGS

17

Glyceraldehyde-3-phosphate dehydrogenase

-1.00

-0.50

-1.00

-0.71

P04406

MASCOT;UNIPROT KB/SWISS-PROT;COGS

18

Voltage-dependent anion-selective channel protein 1

-0.37

-0.19

-0.20

N/A

P21796

MASCOT;UNIPROT KB/SWISS-PROT;COGS

19

Tubulin alpha-8 chain

-0.80

-0.37

N/A

-0.80

Q9NY65

MASCOT;UNIPROT KB/SWISS-PROT;COGS

20

Protein FAM24B precursor

-0.51

-0.54

-0.66

-0.88

Q8N5W8

MASCOT;UNIPROT KB/SWISS-PROT;COGS

21

Interferon alpha-6 precursor

-0.41

N/A

-0.38

-0.75

P05013

MASCOT;UNIPROT KB/SWISS-PROT;COGS

22

ATP-dependent DNA helicase Q5

-1.00

-1.00

-0.93

-0.79

O94762

MASCOT;UNIPROT KB/SWISS-PROT;COGS

23

Sorting nexin-3

-0.25

-1.00

-1.00

-1.00

O60493

MASCOT;UNIPROT KB/SWISS-PROT;COGS

24

Gap junction beta-5 protein

-0.36

-0.16

-0.32

-0.41

O95377

MASCOT;UNIPROT KB/SWISS-PROT;COGS

25

40S ribosomal protein S19

-0.30

-0.30

-0.15

-0.38

P39019

MASCOT;UNIPROT KB/SWISS-PROT;COGS

26

Stress-70 protein, mitochondrial precursor

-0.19

-0.22

N/A

N/A

P38646

MASCOT;UNIPROT KB/SWISS-PROT;COGS

27

Actin-related protein 2/3 complex subunit 5

-1.00

-1.00

-1.00

-1.00

O15511

MASCOT;UNIPROT KB/SWISS-PROT;COGS

28

Sorting nexin-3

-0.36

-0.35

-0.37

-1.00

O60493

MASCOT;UNIPROT KB/SWISS-PROT;COGS

29

Complement receptor type 1 precursor

-0.19

N/A

-0.41

-1.00

P17927

MASCOT;UNIPROT KB/SWISS-PROT;COGS

30

40S ribosomal protein S24

-1.00

-1.00

-1.00

-1.00

P62847

MASCOT;UNIPROT KB/SWISS-PROT;COGS

31

Corticotropin-lipotropin precursor

-0.73

-0.88

-1.00

-1.00

P01189

MASCOT;UNIPROT KB/SWISS-PROT;COGS

32

Inosine triphosphate pyrophosphatase

-1.00

-0.67

-1.00

-1.00

Q9BY32

MASCOT;UNIPROT KB/SWISS-PROT;COGS

33

Peroxiredoxin-1

-0.08

-0.56

-1.00

-0.33

Q06830

MASCOT;UNIPROT KB/SWISS-PROT;COGS

34

Protein memo

-0.95

-0.88

-0.56

-0.47

Q9Y316

MASCOT;UNIPROT KB/SWISS-PROT;COGS

35

Voltage-dependent anion-selective channel protein 2

-1.00

-0.31

-0.46

-0.21

P45880

MASCOT;UNIPROT KB/SWISS-PROT;COGS

36

Thioredoxin-dependent peroxide reductase, mitochondrial precursor

-0.28

-0.19

-0.27

N/A

P30048

MASCOT;UNIPROT KB/SWISS-PROT;COGS

37

RuvB-like 2

-0.24

N/A

-0.43

-0.11

Q9Y230

MASCOT;UNIPROT KB/SWISS-PROT;COGS

38

EF-hand domain-containing protein 2

-0.36

-0.17

-0.37

-0.29

Q5JST6

MASCOT;UNIPROT KB/SWISS-PROT;COGS

39

T-complex protein 1 subunit epsilon

-0.89

N/A

-0.20

-0.97

P48643

MASCOT;UNIPROT KB/SWISS-PROT;COGS

40

Stress-induced-phosphoprotein 1

-0.54

-0.62

-0.44

-0.21

P31948

MASCOT;UNIPROT KB/SWISS-PROT;COGS

41

Retinal dehydrogenase 1

-0.33

-0.79

-0.58

-0.61

P00352

MASCOT;UNIPROT KB/SWISS-PROT;COGS

42

Prostaglandin E synthase 3

-0.26

-0.36

-0.97

-0.16

Q15185

MASCOT;UNIPROT KB/SWISS-PROT;COGS

43

Trypsin-1 precursor

-0.37

-1.00

-0.28

-0.35

P07477

MASCOT;UNIPROT KB/SWISS-PROT;COGS

44

Serpin B9

-0.51

-0.14

-0.21

-0.51

P50453

MASCOT;UNIPROT KB/SWISS-PROT;COGS

45

N-acylneuraminate cytidylyltransferase

-0.64

-0.57

-0.50

-0.28

Q8NFW8

MASCOT;UNIPROT KB/SWISS-PROT;COGS

46

Cofilin-1

-0.76

N/A

-0.85

-0.38

P23528

MASCOT;UNIPROT KB/SWISS-PROT;COGS

47

ADP-ribosylation factor 1

-1.00

-0.21

-0.15

-1.00

P84077

MASCOT;UNIPROT KB/SWISS-PROT;COGS

48

Fructose-bisphosphate aldolase A

-1.00

-1.00

-1.00

-1.00

P04075

MASCOT;UNIPROT KB/SWISS-PROT;COGS

49

Sorting nexin-3

-1.00

-1.00

-1.00

-1.00

O60493

MASCOT;UNIPROT KB/SWISS-PROT;COGS

50

Phosphoglycerate mutase 1

-1.00

-1.00

-1.00

-1.00

P18669

MASCOT;UNIPROT KB/SWISS-PROT;COGS

51

Triosephosphate isomerase

-1.00

-1.00

-1.00

-1.00

P60174

MASCOT;UNIPROT KB/SWISS-PROT;COGS

52

Eukaryotic translation initiation factor 5A-1

-1.00

-1.00

-1.00

-1.00

P63241

MASCOT;UNIPROT KB/SWISS-PROT;COGS

  1. The negative (-) symbol signified suppression of the proteins and a value of 1.00 indicates the complete absence of this protein in the aqueous Phyllanthus-treated sample as compared to the untreated-control sample and the degree of suppression reduces as the value increases. APN – Aqueous P. niruri, APU – Aqueous P. urinaria, APW – Aqueous P. watsonii, APA – Aqueous P. amarus, N/A – Not Affected.