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Table 2 Identified proteins of A549 downregulated (-) in the presence of aqueous Phyllanthus extracts

From: Inhibition of Raf-MEK-ERK and Hypoxia pathways by Phyllanthus prevents metastasis in human lung (A549) cancer cell line

Spot ID Possible proteins Fold change UNIPROT KB/SWISS-PROT Acc. Number Database
APN APU APW APA
1 Actin, cytoplasmic 1 -0.81 -0.88 -0.95 -0.84 P60709 MASCOT;UNIPROT KB/SWISS-PROT;COGS
2 Nicotinamide N-methyltransferase -0.50 N/A -0.28 -0.10 P40261 MASCOT;UNIPROT KB/SWISS-PROT;COGS
3 Alpha-Enolase -0.62 -0.57 -0.48 -0.58 P06733 MASCOT;UNIPROT KB/SWISS-PROT;COGS
4 Proteasome subunit beta type 3 -1.00 -0.20 N/A -1.00 P49720 MASCOT;UNIPROT KB/SWISS-PROT;COGS
5 Retinal dehydrogenase 1 -0.23 N/A -0.13 N/A P00352 MASCOT;UNIPROT KB/SWISS-PROT;COGS
6 Galectin-1 -1.00 -0.13 -0.57 -0.38 P09382 MASCOT;UNIPROT KB/SWISS-PROT;COGS
7 Heat-shock protein beta-1 -0.12 -0.22 -0.32 -0.10 P04792 MASCOT;UNIPROT KB/SWISS-PROT;COGS
8 Glyceraldehyde-3-phosphate dehydrogenase -0.30 -0.93 -0.99 -0.91 P04406 MASCOT;UNIPROT KB/SWISS-PROT;COGS
9 Eukaryotic translation initiation factor 5A-1 -0.32 -0.39 -0.27 -0.80 P63241 MASCOT;UNIPROT KB/SWISS-PROT;COGS
10 60 kDa heat shock protein, mitochondrial precursor -0.46 -0.82 -0.90 -0.75 P10809 MASCOT;UNIPROT KB/SWISS-PROT;COGS
11 Glutathione transferase omega-1 N/A -0.45 N/A -0.22 P78417 MASCOT;UNIPROT KB/SWISS-PROT;COGS
12 Proteasome activator complex subunit 1 -0.19 -0.18 N/A -1.00 Q06323 MASCOT;UNIPROT KB/SWISS-PROT;COGS
13 Annexin A4 -0.29 -0.29 -0.21 -0.65 P09525 MASCOT;UNIPROT KB/SWISS-PROT;COGS
14 Vimentin -0.91 -0.82 -0.24 -0.79 P08670 MASCOT;UNIPROT KB/SWISS-PROT;COGS
15 Peptidyl-prolyl cis-trans isomerase A -0.13 -0.36 -1.00 -0.42 P62937 MASCOT;UNIPROT KB/SWISS-PROT;COGS
16 Peptidyl-prolyl cis-trans isomerase A -1.00 -1.00 -0.32 N/A P62937 MASCOT;UNIPROT KB/SWISS-PROT;COGS
17 Glyceraldehyde-3-phosphate dehydrogenase -1.00 -0.50 -1.00 -0.71 P04406 MASCOT;UNIPROT KB/SWISS-PROT;COGS
18 Voltage-dependent anion-selective channel protein 1 -0.37 -0.19 -0.20 N/A P21796 MASCOT;UNIPROT KB/SWISS-PROT;COGS
19 Tubulin alpha-8 chain -0.80 -0.37 N/A -0.80 Q9NY65 MASCOT;UNIPROT KB/SWISS-PROT;COGS
20 Protein FAM24B precursor -0.51 -0.54 -0.66 -0.88 Q8N5W8 MASCOT;UNIPROT KB/SWISS-PROT;COGS
21 Interferon alpha-6 precursor -0.41 N/A -0.38 -0.75 P05013 MASCOT;UNIPROT KB/SWISS-PROT;COGS
22 ATP-dependent DNA helicase Q5 -1.00 -1.00 -0.93 -0.79 O94762 MASCOT;UNIPROT KB/SWISS-PROT;COGS
23 Sorting nexin-3 -0.25 -1.00 -1.00 -1.00 O60493 MASCOT;UNIPROT KB/SWISS-PROT;COGS
24 Gap junction beta-5 protein -0.36 -0.16 -0.32 -0.41 O95377 MASCOT;UNIPROT KB/SWISS-PROT;COGS
25 40S ribosomal protein S19 -0.30 -0.30 -0.15 -0.38 P39019 MASCOT;UNIPROT KB/SWISS-PROT;COGS
26 Stress-70 protein, mitochondrial precursor -0.19 -0.22 N/A N/A P38646 MASCOT;UNIPROT KB/SWISS-PROT;COGS
27 Actin-related protein 2/3 complex subunit 5 -1.00 -1.00 -1.00 -1.00 O15511 MASCOT;UNIPROT KB/SWISS-PROT;COGS
28 Sorting nexin-3 -0.36 -0.35 -0.37 -1.00 O60493 MASCOT;UNIPROT KB/SWISS-PROT;COGS
29 Complement receptor type 1 precursor -0.19 N/A -0.41 -1.00 P17927 MASCOT;UNIPROT KB/SWISS-PROT;COGS
30 40S ribosomal protein S24 -1.00 -1.00 -1.00 -1.00 P62847 MASCOT;UNIPROT KB/SWISS-PROT;COGS
31 Corticotropin-lipotropin precursor -0.73 -0.88 -1.00 -1.00 P01189 MASCOT;UNIPROT KB/SWISS-PROT;COGS
32 Inosine triphosphate pyrophosphatase -1.00 -0.67 -1.00 -1.00 Q9BY32 MASCOT;UNIPROT KB/SWISS-PROT;COGS
33 Peroxiredoxin-1 -0.08 -0.56 -1.00 -0.33 Q06830 MASCOT;UNIPROT KB/SWISS-PROT;COGS
34 Protein memo -0.95 -0.88 -0.56 -0.47 Q9Y316 MASCOT;UNIPROT KB/SWISS-PROT;COGS
35 Voltage-dependent anion-selective channel protein 2 -1.00 -0.31 -0.46 -0.21 P45880 MASCOT;UNIPROT KB/SWISS-PROT;COGS
36 Thioredoxin-dependent peroxide reductase, mitochondrial precursor -0.28 -0.19 -0.27 N/A P30048 MASCOT;UNIPROT KB/SWISS-PROT;COGS
37 RuvB-like 2 -0.24 N/A -0.43 -0.11 Q9Y230 MASCOT;UNIPROT KB/SWISS-PROT;COGS
38 EF-hand domain-containing protein 2 -0.36 -0.17 -0.37 -0.29 Q5JST6 MASCOT;UNIPROT KB/SWISS-PROT;COGS
39 T-complex protein 1 subunit epsilon -0.89 N/A -0.20 -0.97 P48643 MASCOT;UNIPROT KB/SWISS-PROT;COGS
40 Stress-induced-phosphoprotein 1 -0.54 -0.62 -0.44 -0.21 P31948 MASCOT;UNIPROT KB/SWISS-PROT;COGS
41 Retinal dehydrogenase 1 -0.33 -0.79 -0.58 -0.61 P00352 MASCOT;UNIPROT KB/SWISS-PROT;COGS
42 Prostaglandin E synthase 3 -0.26 -0.36 -0.97 -0.16 Q15185 MASCOT;UNIPROT KB/SWISS-PROT;COGS
43 Trypsin-1 precursor -0.37 -1.00 -0.28 -0.35 P07477 MASCOT;UNIPROT KB/SWISS-PROT;COGS
44 Serpin B9 -0.51 -0.14 -0.21 -0.51 P50453 MASCOT;UNIPROT KB/SWISS-PROT;COGS
45 N-acylneuraminate cytidylyltransferase -0.64 -0.57 -0.50 -0.28 Q8NFW8 MASCOT;UNIPROT KB/SWISS-PROT;COGS
46 Cofilin-1 -0.76 N/A -0.85 -0.38 P23528 MASCOT;UNIPROT KB/SWISS-PROT;COGS
47 ADP-ribosylation factor 1 -1.00 -0.21 -0.15 -1.00 P84077 MASCOT;UNIPROT KB/SWISS-PROT;COGS
48 Fructose-bisphosphate aldolase A -1.00 -1.00 -1.00 -1.00 P04075 MASCOT;UNIPROT KB/SWISS-PROT;COGS
49 Sorting nexin-3 -1.00 -1.00 -1.00 -1.00 O60493 MASCOT;UNIPROT KB/SWISS-PROT;COGS
50 Phosphoglycerate mutase 1 -1.00 -1.00 -1.00 -1.00 P18669 MASCOT;UNIPROT KB/SWISS-PROT;COGS
51 Triosephosphate isomerase -1.00 -1.00 -1.00 -1.00 P60174 MASCOT;UNIPROT KB/SWISS-PROT;COGS
52 Eukaryotic translation initiation factor 5A-1 -1.00 -1.00 -1.00 -1.00 P63241 MASCOT;UNIPROT KB/SWISS-PROT;COGS
  1. The negative (-) symbol signified suppression of the proteins and a value of 1.00 indicates the complete absence of this protein in the aqueous Phyllanthus-treated sample as compared to the untreated-control sample and the degree of suppression reduces as the value increases. APN – Aqueous P. niruri, APU – Aqueous P. urinaria, APW – Aqueous P. watsonii, APA – Aqueous P. amarus, N/A – Not Affected.