Figure 3

Neuroprotective effect of ethyl acetate fraction from aged garlic extract on Aβ-induced cytotoxicity in PC12 cells. (A) Protective effect of the ethyl acetate fraction on Aβ-induced cytotoxicity in PC12 cell system. PC12 cells were pretreated for 24 h with various concentrations. After 24 h, the cells were treated with 80 μM Aβ for 3 h. (B) Inhibitory effect of the ethyl acetate fraction on Aβ-induced membrane damage in PC12 cells. PC12 cells were pretreated for 48 h with various concentrations. After 48 h, the cells were treated with 80 μM Aβ for 3 h. Each value represented the means ± SD of triplicates. Significant difference (p < 0.05 versus vitamin C) was observed on the Aβ-induced cell death.