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Table 1 MS/MS analysis and characteristics of the analyzed spots

From: Application of proteomics to identify the target molecules involved in Lonicera japonica-induced photokilling in human lung cancer CH27 cells

Spot      Protein Accession no. Calculated pI Theoretical pI/Mw (Da) Score Sequence coverage (%) Changed fold (% control)1
D1  DJ-1 protein NP_009193 6.33 6.32 / 19891.05 88 25 40 ± 4
D2  Heat shock protein 27 AAA62175 6.16 5.98 / 22782.52 273 43 46 ± 5
D3  Protein disulfide isomerase CAA89996 5.85 5.98 / 56782.39 766 40 48 ± 9
D4  Heat shock 70 kDa protein 9 AAH00478 5.59 5.87 / 73680.50 508 28 49 ± 7
D5  Actin, cytoplasmic 1 NP_001092 5.42 5.29 / 41736.73 896 43 41 ± 5
D6  ATP synthase NP_006347 5.21 5.21 / 18491.21 147 34 48 ± 3
D7  Tropomyosin 3 isoform 2 NP_705935 4.81 4.68 / 32818.79 283 29 45 ± 11
D8  Tropomyosin 3 isoform 4 NP_001036816 4.73 4.73 / 28870.36 283 29 79 ± 5
#1  Protein disulfide isomerase CAA89996 5.88 5.98 / 56782.39 423 32 383 ± 26
#2  Protein disulfide isomerase CAA89996 5.70 5.98 / 56782.39 494 32 143 ± 21
#3  Heat shock 70 kDa protein 9B precursor variant BAD96478 5.60 5.87 / 73680.50 405 25 1594 ± 115
#4  Heat shock 70 kDa protein 8 isoform 1 NP_006588 5.43 5.61 / 53499.58 540 28 366 ± 62
#5  Chaperonin NP_002147 5.34 5.70 / 61054.64 760 46 145 ± 25
#6  Chaperonin NP_002147 5.15 5.70 / 61054.64 600 31 386 ± 51
#7  Actin, cytoplasmic 1 NP_001092 5.25 5.29 / 41736.73 336 37 266 ± 10
#8  Actin, cytoplasmic 1 NP_001092 5.15 5.29 / 41736.73 262 39 376 ± 43
  1. 1The expression of protein spots was quantified by AlphaEase image software. All protein changed folds are expressed as the mean percentage of the relative control ± S.D. from three independent experiments.