Figure 5

Effects of TPA and STE on the phosphorylation level of Src and FAK. (A) HONE-1 cells were treated with TPA (50 ng/mL) for 15, 30, 60, and 120 min and then subjected to Western blotting to analyze Src and FAK levels. (B) HONE-1 cells were treated with STE (0, 50, and 100 μg/mL) in the presence or absence of TPA (50 ng/mL) for 6 h and then subjected to Western blotting to to analyze Src and FAK levels. Quantitative results of phosphorylation levels of Src or FAK were adjusted with total Src and FAK protein level. The values represented the means±SD of at least three independent experiments. *p< 0.05 compared to the vehicle group; #p< 0.05 compared to the TPA treatment group.