Figure 3

Calcarea carbonica normalizes cytokine-producing effector T cell populations in tumor-bearing host. (A) Typical flow cytometric patterns for IFN-γ and IL-4 producing CD4⁺ T cells in draining lymph nodes of mice. Graphical representation of flow cytometric data of intracellular IFN-γ (B), IL-4 (C) and type-2 cytokine bias ratio (D) in CD4⁺/CD8⁺ T cells of thymus, spleen, lymph node and peripheral circulation of untreated or placebo-/calcarea carbonica-treated tumor-bearing and normal mice at 21 days of drug administration. Values are mean ±SEM of five independent experiments. *p < 0.05 and **p < 0.001 when compared with respective non-tumor/tumor bearing control sets and tumor bearing placebo/drug-treated sets.