Figure 2From: The standardized herbal formula, PM014, ameliorated cigarette smoke-induced lung inflammation in a murine model of chronic obstructive pulmonary disease Cellular profiles in BAL fluid and microscopic findings of the intrapulmonary bronchi. After sacrificing the mice, PBS buffer was slowly infused into the lungs and withdrawn via a cannula inserted into the trachea. The cell numbers were counted using a hemacytometer, and differential cell counts were performed on slides prepared by cytocentrifugation at 250 rpm for 3 min and Diff-Quick staining. A) Count of total cell number, B) Count of neutrophils, C) Count of macrophages, D) Count of lymphocytes. Data are shown as mean ± S.E.M. Statistical analyses were conducted by one-way ANOVA followed by Newman-Keuls Multiple Comparison test (***p < 0.001, **p < 0.01 vs. CON, ###p < 0.001, ##p < 0.01, #p < 0.05 vs. CS; n = 5–6).Back to article page