Figure 2From: Estrogenic and mutagenic activities of Crotalaria pallida measured by recombinant yeast assay and Ames test Estrogenic response for extract dichloromethane fraction of C. pallida , in the recombinant yeast assay. Different concentrations of dichloromethane fraction of C. pallida, (μg/well) were added to genetically engineered, estrogen- responsive yeast cells and incubated for 6 h. The β-galactosidase activities were calculated as fluorescence units (FU). Values are averages of three independent experiments; bars indicate value ranges. Negative control, DMSO, FU = 432 ± 34; positive control, 17-β-estradiol, FU = 11490 ± 496.Back to article page