Figure 1

Morphological changes of HDFs in culture. Young (a), pre-senescent (b) senescent HDFs (c), senescent HDFs treated for 24 h with 0.2 mg/ml aqueous extract of P. betle (d), 0.1 mg/ml hot water extract of C. vulgaris (e), and 0.5 mg/ml TRF (f). Cells expressing SA β-gal were stained blue (white arrows). Micrographs are shown at 100× magnification.