Representative photomicrograph of IMR-32 cells.(A) after pre-treated with LY294002 or PD098059 in the presence or absence of ECa 233 (1, 10 and 100 μg/ml) (scale bar = 50 μm). (B) Morphometric analysis of the cell treated with LY294002 or PD098059 on the increment of neurite outgrowth induced by ECa 233. Neurites from 60 living cells per treated condition were evaluated, (n = 3). Data are presented as mean ± S.E., ** = p < 0.001 vs non-treated control. ## = p < 0.001 vs ECa 233 treated groups.