Relative blot density of pERK/ERK and pAkt/Akt in response to ECa 233 treatment.(A) ERK1/2 and ERK1/2 phosphorylation (B) Akt and Akt phosphorylation. The level of phospho-ERK1/2 and phospho-Akt in IMR-32 cells after treated with ECa 233 (1, 10 and 100 μg/ml) for 6 h were significantly higher than that of control. Densitometric analysis of the indicated proteins normalized with the density of GAPDH band were plated and presented as mean ± S.D. of three independent experiments, * = p < 0.05, ** = p < 0.001 vs non-treated control.