Figure 4

Immunoblotting for β-Catenin, C-myc, Cyclin D1 and Survival proteins using lysates from MCF-7 (A) or MDA-MB-231 (B) cells treated with DMSO (control) or 5 and 10 μmol/L GL standardized to z-Gug or 10 and 40 μmol/L z-Gug for 24 h. The blot was stripped and reprobed with anti-actin antibody to ensure equal protein loading. The numbers on top of the immunoreactive bands represent change in protein levels relative to corresponding DMSO-treated control. Immunoblotting for β-Catenin protein using isolated cytosolic and nuclear fractions from MCF-7 (C) or MDA-MB-231 (D) cells following 24-h treatment with DMSO or 5 μmol/L GL or 40 μmol/L z-Gug. The blot was reprobed with anti-α-Tubulin or anti-PARP antibody to ensure purity of the nuclear fraction. The numbers on top of the immunoreactive bands represent change in levels relative to DMSO-treated control. Immunoblotting for each protein was performed at least twice using independently prepared lysates.