Table 1 Primers sequence of genes for quantitative PCR
Gene Name | GenBank Accession Number | Primer Sequence 5′-3′ | PCR product size (bp) |
|---|---|---|---|
GAPDH | NM_002046 | F: 5′-tcc ctg agc tga acg gga ag-3′ | 217 |
R: 5′-gga gga gtg ggt gtc gct gt-3′ | |||
COL I | NM_000088 | F: 5′-agg gct cca acg aga tcg aga-3′ | 222 |
R: 5′-tac agg aag cag aca ggg cca-3′ | |||
COL II | NM_001844 | F: 5′-cta tct gga cga agc agc tgg ca-3′ | 209 |
R: 5′-atg ggt gca atg tca atg atgg-3′ | |||
ACP | NM_001135 | F: 5′-gcg gag gaa gtc ggt gaa ga-3′ | 183 |
R: 5′-ccc tct cgc ttc agg tca gc-3′ | |||
SOX-9 | NM_000346 | F: 5′-cac tgt tac cgc cac ttc cc-3′ | 236 |
R: 5′-acc agc gga agt ccc ctt cg-3′ | |||
IL-1 | NM_000576 | F: 5′-gga caa gct gag gaa gat gc-3′ | 120 |
R: 5′-tcg tta tcc cat gtg tcg aa-3′ | |||
IL-6 | NM_000600 | F: 5′-tac ccc cag gag aag att cc-3′ | 175 |
R: 5′-ttt tct gaa agt gcc tct tt-3′ | |||
IL-8 | NM_000584 | F: 5′-gtg cag ttt tgc caa gga gt-3′ | 196 |
R: 5′-ctc tgc acc cag ttt tcc tt-3′ | |||
MMP1 | NM_002421 | F: 5′-agg gtt gaa aag cat gag ca-3′ | 111 |
R: 5′-ctg gtt gaa aag cat gag ca-3′ | |||
MMP3 | NM_002422 | F: 5′-tgc ttt gtc ctt tga tgc tg-3′ | 135 |
R: 5′-gga aga gat ggc caa aat ga-3′ | |||
MMP13 | NM_002427 | F: 5′-ggt ctt gac cac tcc aag gac-3′ | 221 |
R: 5′-ctc ctc gga gac tgg taa tgg-3′ | |||
iNOS | NM_000625 | F: 5′-aca agc cta ccc ctc cag at-3′ | 158 |
R: 5′-tcc cgt cag ttg gta ggt tc-3′ | |||
COX-2 | NM_000963 | F: 5′-tga gca tct acg gtt tgc tg-3′ | 158 |
R: 5′-tgc ttg tct gga aca act gc-3′ |