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Figure 8 | BMC Complementary and Alternative Medicine

Figure 8

From: Induction of apoptosis in melanoma A375 cells by a chloroform fraction of Centratherum anthelminticum (L.) seeds involves NF-kappaB, p53 and Bcl-2-controlled mitochondrial signaling pathways

Figure 8

Caspase activities in CACF-treated cells. (A) Caspase-3/7, -8 and -9 activities in the CACF (12.5 μg/mL)-treated A375 cells were measured in luminescence against various time intervals. Activity of caspase-9 was increased followed by caspase-3/7 activities after CACF treatment. (B) A375 cells were pretreated with inhibitors for pan-caspase (Z-VAD-FMK), caspase-3 (Z-DEVD-FMK), caspase-9 (Z-LEHD-FMK) or caspase-8 (Z-IETD-FMK) before adding CACF. Caspase-3/7 activities in CACF-treated cells were then determined by luminescence assays. Data were mean ± SD of fluorescence intensity readings measured from different photos taken (*P<0.05).

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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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