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Figure 4 | BMC Complementary and Alternative Medicine

Figure 4

From: Induction of apoptosis in melanoma A375 cells by a chloroform fraction of Centratherum anthelminticum (L.) seeds involves NF-kappaB, p53 and Bcl-2-controlled mitochondrial signaling pathways

Figure 4

Cell apoptosis upon CACF treatment. (A) A375 cells were treated with DMSO or with various concentrations of CACF for 24 h. Cells were then stained with FITC-Annexin V and PI to analyze early apoptotic (Annexin V+ PI-) and late apoptotic/dead (Annexin V+ PI+) cell fractions through flow cytometry. (B) Histogram shows the percentages of apoptotic cells for each treated sample at 24 h after CACF treatment. Data were mean ± SD from 2 independent experiments (*P<0.05). (C) Transmission electron microscopy images of A375 cells untreated or treated with 12.5 μg/ml of CACF for 12 h. (C-I) Representative image of untreated control cells. Intact cell membrane and abundant mitochondria were seen in the cytoplasm. (C-II-IV) Representative images of CACF (12.5 μg/mL)-treated cells. (C-II) Plasma membrane (PM) blebing and nuclear chromatin (CHR) condensation. (C-III) Increased numbers of clear vacuoles (V) with some lipid-filled vacuoles (LV). Reduced mitochondria copy number and disrupted cristae in the abnormally swollen mitochondria. (C-IV) Increased numbers of lysosome (LY) organelles.

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