Figure 3
From: Potentiation of neuritogenic activity of medicinal mushrooms in rat pheochromocytoma cells
Effects of the specific inhibitors of MEK/ERK1/2 and PI3K on aqueous extracts-stimulated neuritogenesis. PC-12 cells were exposed to U0126, PD98059 and LY294002 for 1 h before the treatment of aqueous extracts of G. lucidum (GL), G. neo-japonicum (GNJ) and G. frondosa (GF). Cells in complete F-12 K medium without treatment served as a negative control. Cells treated with 50 ng/ml of NGF or 50 μg/ml of H. erinaceus (HE) served as positive controls. A control without inhibitor was used in each treatment group. Data were expressed as means ± standard deviation (n = 3). *** p < 0.001 compared to respective controls.