Figure 2

Effect of EESP on tumor growth in colorectal cancer (CRC) xenograft mice and HT-29 colon cancer cells. After tumor development, the mice were given intra-gastric administration of 6 g/kg/d dose of EESP or saline daily, 5 days per week for 16 days. Tumor volume (A), tumor weight (B) and body weight (C) were was measured at the end of experiment. (D) HT-29 cell viability was determined by the MTT assay after cells were treated with the indicated concentrations of EESP for 24 h. The data were normalized to the viability or survival of control cells (100%, treated with 0.5% DMSO vehicle). Data shown are averages with S.D. (error bars) from 9 individual mice in each group or from three independent cell-based experiments. *P< 0.01, versus controls.