Table 1 The sequence, T m and amplicon size of the designed primers used in PCR
From: Effect of black tea extract on herpes simplex virus-1 infection of cultured cells
Primers | Target genes | Nucleotide sequence (5′ to 3′) | Tm(°C) | Amplicon (nt) |
|---|---|---|---|---|
gD1F | HSV-1 US6 | AGACGTCCGGAAACAACCCTACAA | 64.6 | 752 |
gD1R | ACACAATTCCGCAAATGACCAGGG | 64.6 | ||
GFPF | HSV-1 GFP | TGACCCTGAAGTTCATCTGCACCA | 64.6 | 717 |
GFPR | AACTCCAGCAGGACCATGTGAT | 62.7 | ||
VP1F | HSV-1 UL46 | ACCAAGCCTTGATGCTCAACTCCA | 64.6 | 957 |
VP1R | ACAACACGGTTCCCGAGAGTTTGA | 64.6 |