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Table 4 Identification by nano HPLC-ESI-MS/MS of S. mutans cytosol proteins differentially expressed when treated with 2x LMM fraction of mushroom extract

From: Effects of mushroom and chicory extracts on the shape, physiology and proteome of the cariogenic bacterium Streptococcus mutans

Protein name Spot no. Fold of variation NCBI acc. # Gene name Theor. Mr. (Da) Theor. pI No. of peptides identified Mascot scorea) Sequence coverageb)(%) Molecular function
STRESS RESPONSE
60 kDa chaperonin 1605 + 2.17 gi|21666296 groEL 56428 4.68 39 2392 78 Protein binding
Molecular chaperone dnaK 1706 + 2.29 gi|24378606 dnaK 65246 4.58 28 1265 51 Protein binding
10 kDa chaperonin 3001 + 3.25 gi|21666295 groES 10064 4.93 4 273 32 Protein binding
Superoxide dismutase 4108 + 2.60 gi|24379102 sodA 22611 4.99 9 521 56 Ion binding
Glutathione reductase 6607 + 3.89 gi|4587160 gshR 48955 5.40 3 143 7 Oxidoreductase activity
SUGAR BIOSYNTHESIS AND METABOLISM
Glucose-1-phosphate adenylyltransferase 2501 - 3.57 gi|92081399 glgC 41906 4.72 13 680 38 Transferase activity
Phosphopyruvate hydratase 2806 - 2.56 gi|24379669 eno 46829 4.67 14 771 40 Lyase activity
Putative phosphoglucomutase 3703 - 12.50 gi|24379514 pgm 63056 4.88 33 1783 69 Transferase activity
Tagatose 1,6-diphosphate aldolase 1 4303 - 2.17 gi|153676 lacD1 36468 4.96 11 462 31 Lyase activity
Putative N-acetylglucosamine-6-phosphate isomerase 6205 + 2.43 gi|24379109 SmuNN2025_1351 25457 5.33 7 330 37 Deaminase activity
Fructose bi-phosphate aldolase 8001 + 5.24 gi|4322370 fba 7447 8.85 3 115 30 Lyase activity
PROTEIN BIOSYNTHESIS AND FOLDING
Elongation factor Tu 1603 - 2.44 gi|24379182 tuf 43891 4.84 25 1257 72 GTPase activity
Trigger factor 1701 + 2.06 gi|24378614 tig 47457 4.46 20 918 51 Isomerase activity
50S ribosomal protein L4 3101 + 2.18 gi|254998187 SmuNN2025_1762 22215 9.76 6 313 39 RNA binding
Elongation factor Ts 3503 - 2.38 gi|24380373 tsf 37695 4.91 22 1218 74 GTP binding
CELL REDOX HOMEOSTASIS
Thiol peroxidase 0007 + 4.33 gi|254997527 tpx 17541 4.56 8 450 34 Oxidoreductase activity
Alkyl hydroperoxide reductase 1107 + 12.60 gi|24379223 ahpC 20465 4.57 6 284 34 Oxidoreductase activity
Putative bacterocin transport accessory protein, Bta 5002 + 2.71 gi|24380154 SMU_1788c 12601 5.21 2 85 18 Oxidoreductase activity
Putative glutathione S-transferase 8202 + 5.64 gi|24379712 SMU_1296 29973 5.74 5 243 16 Transferase activity
Not identified 8007 - 3.57         
  1. a) Score is –106log(p), where p is the probability that the observed match is a random event, based on the NCBI database using the MASCOT searching program as MS/MS data.
  2. b) Amino acid sequence coverage for the identified protein.