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Table 4 Identification by nano HPLC-ESI-MS/MS of S. mutans cytosol proteins differentially expressed when treated with 2x LMM fraction of mushroom extract

From: Effects of mushroom and chicory extracts on the shape, physiology and proteome of the cariogenic bacterium Streptococcus mutans

Protein name

Spot no.

Fold of variation

NCBI acc. #

Gene name

Theor. Mr. (Da)

Theor. pI

No. of peptides identified

Mascot scorea)

Sequence coverageb)(%)

Molecular function

STRESS RESPONSE

60 kDa chaperonin

1605

+ 2.17

gi|21666296

groEL

56428

4.68

39

2392

78

Protein binding

Molecular chaperone dnaK

1706

+ 2.29

gi|24378606

dnaK

65246

4.58

28

1265

51

Protein binding

10 kDa chaperonin

3001

+ 3.25

gi|21666295

groES

10064

4.93

4

273

32

Protein binding

Superoxide dismutase

4108

+ 2.60

gi|24379102

sodA

22611

4.99

9

521

56

Ion binding

Glutathione reductase

6607

+ 3.89

gi|4587160

gshR

48955

5.40

3

143

7

Oxidoreductase activity

SUGAR BIOSYNTHESIS AND METABOLISM

Glucose-1-phosphate adenylyltransferase

2501

- 3.57

gi|92081399

glgC

41906

4.72

13

680

38

Transferase activity

Phosphopyruvate hydratase

2806

- 2.56

gi|24379669

eno

46829

4.67

14

771

40

Lyase activity

Putative phosphoglucomutase

3703

- 12.50

gi|24379514

pgm

63056

4.88

33

1783

69

Transferase activity

Tagatose 1,6-diphosphate aldolase 1

4303

- 2.17

gi|153676

lacD1

36468

4.96

11

462

31

Lyase activity

Putative N-acetylglucosamine-6-phosphate isomerase

6205

+ 2.43

gi|24379109

SmuNN2025_1351

25457

5.33

7

330

37

Deaminase activity

Fructose bi-phosphate aldolase

8001

+ 5.24

gi|4322370

fba

7447

8.85

3

115

30

Lyase activity

PROTEIN BIOSYNTHESIS AND FOLDING

Elongation factor Tu

1603

- 2.44

gi|24379182

tuf

43891

4.84

25

1257

72

GTPase activity

Trigger factor

1701

+ 2.06

gi|24378614

tig

47457

4.46

20

918

51

Isomerase activity

50S ribosomal protein L4

3101

+ 2.18

gi|254998187

SmuNN2025_1762

22215

9.76

6

313

39

RNA binding

Elongation factor Ts

3503

- 2.38

gi|24380373

tsf

37695

4.91

22

1218

74

GTP binding

CELL REDOX HOMEOSTASIS

Thiol peroxidase

0007

+ 4.33

gi|254997527

tpx

17541

4.56

8

450

34

Oxidoreductase activity

Alkyl hydroperoxide reductase

1107

+ 12.60

gi|24379223

ahpC

20465

4.57

6

284

34

Oxidoreductase activity

Putative bacterocin transport accessory protein, Bta

5002

+ 2.71

gi|24380154

SMU_1788c

12601

5.21

2

85

18

Oxidoreductase activity

Putative glutathione S-transferase

8202

+ 5.64

gi|24379712

SMU_1296

29973

5.74

5

243

16

Transferase activity

Not identified

8007

- 3.57

        
  1. a) Score is –106log(p), where p is the probability that the observed match is a random event, based on the NCBI database using the MASCOT searching program as MS/MS data.
  2. b) Amino acid sequence coverage for the identified protein.