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Table 3 Identification by nano HPLC-ESI-MS/MS of S. mutans membrane proteins differentially expressed when treated with 2x LMM fraction of mushroom extract

From: Effects of mushroom and chicory extracts on the shape, physiology and proteome of the cariogenic bacterium Streptococcus mutans

Protein name Spot no. Fold of variation NCBI acc. # Gene name Theor. Mr. (Da) Theor. pI No. of peptides identified Mascot scorea) Sequence coverageb)(%) Molecular function
MEMBRANE TRANSPORT
F0F1 ATP synthase subunit delta 2202 - 2.27 gi|24379920 atpH 20436 5.22 9 528 51 ATPase activity
Putative ABC transporter, ATP-binding protein 5303 - 2.08 gi|24380277 psaA 26297 5.58 12 694 57 ATPase activity
Putative oligopeptide ABC transporter, ATP-binding protein 5601 - 2.38 gi|24378766 oppD 39147 5.47 15 680 56 ATPase activity
Putative sugar ABC transporter, ATP-binding protein 5804 - 7.32 gi|24379552 SMU_1120 55575 5.78 26 1197 62 ATPase activity
Putative amino acid ABC transporter, ATP-binding protein 6406 - 2.56 gi|24378949 SMU_461 27674 5.83 19 847 75 ATPase activity
Putative amino acid ABC transporter, ATP-binding protein 7303 - 9.09 gi|24379380 SMU_936 28340 5.90 2 118 10 ATPase activity
Putative phosphate ABC transporter, ATP-binding protein 7304 - 2.17 gi|24379566 pstB 30276 5.90 3 136 12 ATP binding
Multiple sugar-binding ABC transporter, ATP-binding protein 7702 - 2.94 gi|24379336 msmK 41938 5.92 14 579 39 ATPase activity
SUGAR BIOSYNTHESIS AND METABOLISM
Putative glucose-1-phosphate thymidyltransferase 2402 - 2.50 gi|24379858 rmlA 32275 4.90 16 917 53 Tranferase activity
Pyruvate kinase 4101 + 2.76 gi|24379618 pykF 54333 5.09 2 70 5 Kinase activity
Glyceraldehyde-3-phosphate dehydrogenase 4601 + 2.28 gi|24378857 gapC 36046 5.71 17 725 44 Oxidoreductase activity
Galactose 6-P isomerase 6102 + 3.01 gi|153674 lacA 19025 5.80 2 84 15 Isomerase activity
Catabolite control protein A 8501 - 6.14 gi|2155300 ccpA 36590 7.08 3 124 9 DNA binding
PROTEIN BIOSYNTHESIS AND FOLDING
60 kDa chaperonin 5102 + 2.19 gi|13898679 cpn60 19827 4.53 2 87 14 Protein binding
Phenylalanyl-tRNA synthetase subunit alpha 6603 - 7.83 gi|24379902 pheS 39271 5.87 14 420 34 Nucleotide binding
30S ribosomal protein S8 9103 + 2.24 gi|24380355 rpsH 14684 9.10 7 411 64 RNA binding
CELL CYCLE AND DIVISION
Putative Hit-like protein 2102 + 2.07 gi|24378905 SMU_412c 15560 5.13 3 139 30 Unknown
Hypothetical protein SMU.471 4002 - 2.04 gi|24378958 SMU471 12972 5.37 5 255 42 Methylase activity
UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1 5702 + 2.30 gi|24379914 murA1 45588 5.65 10 422 30 Tranferase activity
AMINOACIDS BIOSYNTHESIS AND METABOLISM
Uridylate kinase 3302 + 4.08 gi|24380005 pyrH 26315 5.48 9 460 52 Kinase activity
Phospho-2-dehydro-3-deoxyheptonate aldolase 4505 + 2.60 gi|24380198 aroG 39072 6.02 8 338 29 Lyase activity
Shikimate 5-dehydrogenase 5401 + 2.36 gi|24379237 aroE 31791 6.19 2 106 11 Oxidoreductase activity
Putative D-3-phosphoglycerate dehydrogenase 5604 - 3.33 gi|24380031 serA 42771 5.56 14 815 51 Oxidoreductase activity
MISCELLANEOUS
Putative dihydrolipoamide dehydrogenase 3603 + 4.23 gi|24378648 adhD 61708 4.88 4 270 10 Oxidoreductase activity
Orotate phosphoribosyltransferase 4201 + 2.38 gi|24379645 pyrE 22802 5.37 7 381 45 Glycosyltransferase activity
Putative exodeoxyribonuclease III 6403 - 2.56 gi|24380027 smnA 31393 5.72 11 558 44 DNA binding
(3R)-hydroxymyristoyl-ACP dehydratase 7403 - 2.08 gi|24380109 fabZ 15361 8.83 3 150 21 Lyase activity
Not identified 5301 + 4.52         
Not identified 8205 + 2.59         
Not identified 8401 - 4.35         
Not identified 8506 + 3.30         
  1. a) Score is –106log(p), where p is the probability that the observed match is a random event, based on the NCBI database using the MASCOT searching program as MS/MS data.
  2. b) Amino acid sequence coverage for the identified protein.