Figure 1
From: Water extract of Spatholobus suberectus inhibits osteoclast differentiation and bone resorption
Inhibitory effect of WESS on osteoclast differentiation in bone marrow cell-osteoblast coculture. Mouse bone marrow cells and calvarial osteoblasts were cocultured with vehicle (DW) or WESS in the presence of VitD3 (10 nM) for 6 days. (A) Representative microscopic pictures of TRAP staining are shown at 100× magnification. (B) The number of TRAP-positive multinucleated osteoclasts (TRAP(+)MNC) containing three or more nuclei were counted. Data represents mean ± SD of three independent experiments. c P < 0.001 vs. vehicle-treated control group. (C) Osteoblasts were pre-incubated with vehicle or WESS (25 μg/ml) for 3 h and stimulated with VitD3 (10 nM) for 24 h. RANKL and OPG mRNA levels were analyzed by QPCR.