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Table 1 Expression changes of estrogen-responsive genes in MCF-7 cells treated with E2 and SWT

From: Transcriptional profiling of Chinese medicinal formula Si-Wu-Tang on breast cancer cells reveals phytoestrogenic activity

  Fold change (treatment/control)
Affymetrix probe ID Gene symbol Description E2 SH SM
Up-regulated genes
237460_x_at C14orf182 chromosome 14 open reading frame 182 13.77 6.29 1.18
228554_at PGR progesterone receptor 12.10 2.83 1.72
205862_at GREB1 growth regulation by estrogen in breast cancer 1 10.87 5.08 2.37
206115_at EGR3 early growth response 3 10.69 10.70 1.65
235004_at RBM24 RNA binding motif protein 24 10.23 6.07 1.57
1557277_a_at    8.71 2.08 1.32
231120_x_at PKIB protein kinase (cAMP-dependent, catalytic) inhibitor beta 7.96 2.29 1.27
219525_at SLC47A1 solute carrier family 47, member 1 7.93 2.22 1.40
205440_s_at NPY1R neuropeptide Y receptor Y1 7.08 2.81 1.84
44790_s_at C13orf18 chromosome 13 open reading frame 18 7.08 2.97 2.59
213906_at MYBL1 v-myb myeloblastosis viral oncogene homolog (avian)-like 1 6.78 1.26 1.29
220038_at SGK3 serum/glucocorticoid regulated kinase family, member 3 6.63 1.79 1.51
222921_s_at HEY2 hairy/enhancer-of-split related with YRPW motif 2 6.11 2.88 1.59
228241_at AGR3 anterior gradient homolog 3 (Xenopus laevis) 6.08 2.55 1.84
204798_at MYB v-myb myeloblastosis viral oncogene homolog (avian) 5.96 2.37 1.92
227627_at SGK3 serum/glucocorticoid regulated kinase family, member 3 5.83 1.69 1.54
208018_s_at HCK hemopoietic cell kinase 5.77 1.33 1.02
239777_at C14orf182 chromosome 14 open reading frame 182 5.63 3.29 1.27
207886_s_at CALCR calcitonin receptor 5.38 2.59 1.86
232306_at CDH26 cadherin 26 5.37 3.51 1.94
219743_at HEY2 hairy/enhancer-of-split related with YRPW motif 2 5.30 1.83 1.37
208305_at PGR progesterone receptor 5.22 1.69 1.10
223551_at PKIB protein kinase (cAMP-dependent, catalytic) inhibitor beta 5.18 2.13 1.42
211421_s_at RET ret proto-oncogene 4.98 1.54 1.19
205326_at RAMP3 receptor (G protein-coupled) activity modifying protein 3 4.67 2.35 1.87
244745_at RERG RAS-like, estrogen-regulated, growth inhibitor 4.61 1.43 1.51
237334_at    4.55 2.25 1.34
219702_at PLAC1 placenta-specific 1 4.45 2.02 1.09
223201_s_at TMEM164 transmembrane protein 164 4.44 1.68 1.23
219825_at CYP26B1 cytochrome P450, family 26, subfamily B, polypeptide 1 4.42 1.26 1.19
224428_s_at CDCA7 cell division cycle associated 7 4.39 1.11 1.45
209687_at CXCL12 chemokine (C-X-C motif) ligand 12 4.31 1.77 1.29
242064_at SDK2 sidekick homolog 2 (chicken) 4.25 1.48 0.99
219985_at HS3ST3A1 heparan sulfate (glucosamine) 3-O-sulfotransferase 3A1 4.17 2.01 1.40
220486_x_at TMEM164 transmembrane protein 164 4.09 1.64 1.22
201739_at SGK1 serum/glucocorticoid regulated kinase 1 4.01 1.86 1.29
Down-regulated genes
242943_at ST8SIA4 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 0.31 0.40 0.85
202948_at IL1R1 interleukin 1 receptor, type I 0.32 0.40 0.78
229354_at AHRR aryl-hydrocarbon receptor repressor 0.33 0.83 0.80
230261_at ST8SIA4 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 0.36 0.41 0.88
230836_at ST8SIA4 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 0.37 0.35 0.88
228176_at EDG3 endothelial differentiation, sphingolipid G-protein-coupled receptor, 3 0.38 0.67 0.95
205749_at CYP1A1 cytochrome P450, family 1, subfamily A, polypeptide 1 0.38 1.76 0.97
213413_at BG434174 STON1 0.39 0.27 0.63
  1. Gene expression of MCF-7 cells after 6 hours of treatment with 0.1 μM E2 or SWT (2.56, 0.256 or 0.0256 mg/ml) was analyzed by microarrays. Listed are selected genes that showed strongest up-regulation or down-regulations induced by E2 by applying the cutoff of fold change > 4 for up-regulated genes, fold change < 0.4 for down-regulated genes, and false discovery rate (FDR) < 0.01. Expression values are shown by fold changes between treatment and control, i.e., expression values relative to untreated control cells for E2, SH and SM. Fold changes in bold: FDR < 0.01.