Figure 4From: The anticancer effect of saffron in two p53 isogenic colorectal cancer cell lines Saffron induced DNA damage and autophagy.(A) Western Blot analysis for caspase 3 and PARP cleavage in two cell types, HCT116 p53wildtype and HCT116 p53 −/− cells, untreated (Ctrl) or treated with 3 mg/ml saffron for 6, 24, and 48 h. (B) Western Blot analysis for γH2AX expression in HCT116 p53 wildtype and HCT116 p53 −/− cells, untreated (Ctrl) or treated with 3 mg/ml saffron for 6, 24, and 48 h. (C) Western Blot analysis for LC3 expression in HCT116 p53 wildtype and HCT116 p53 −/− cells, untreated (Ctrl) or treated with 3 mg/ml saffron for 6, 24, and 48 h. (D) Western Blot analysis for Beclin 1 expression in HCT116 p53 wildtype and HCT116 p53 −/− cells, untreated (Ctrl) or treated with 3 mg/ml saffron for 6, 24, and 48 h.Back to article page