Figure 2
From: The anticancer effect of saffron in two p53 isogenic colorectal cancer cell lines
Saffron induced cell cycle arrest in HCT116 cells.(A) Two cell types, HCT116 p53 wildtype and HCT116 p53 −/− cells, untreated (Ctrl) or treated with 3 mg/ml saffron for 24 and 48 h, were harvested and DNA was stained with PI for flow cytometric analysis of DNA content with FACScan flow cytometry. (B) Quantitative analysis of percentage gated cells at PreG1, G1, S and G2 phases in the HCT116 p53 wildtype and HCT116 p53 −/− cells. Results are means ± s.d. (n = 3). (C) Western Blot analysis for Cyclin B1, Histone 3, and phosphoHistone 3 (pH3) expressions in HCT116 p53 wildtype and HCT116 p53 −/− cells, untreated (Ctrl) or treated with 3 mg/ml saffron for 6, 24, and 48 h. (*, Saffron treatment was statistically significant against the control for P < 0.05).