Figure 1

The experimental design for measuring the ascorbic acid mediated cytotoxic effect. (A) Cells in logarithmic growth phase were exposed to serial dilutions of ascorbic acid (5-100 mmol/L) for 14 h (exposure time). Afterwards, the cells were washed and cultured in cultured medium free of ascorbic acid, for 10, 34 and 58 h. (B) The number of viable cells was measured after culture by crystal violet (CV) staining with an ELISA reader.