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Table 4 The percentage scavenging activity of the crude DCM extract of bee pollen (CDEb) and its subfractions (CDEb1 - CDEb7) following silica gel 60 quick column chromatography

From: Chemical constituents and free radical scavenging activity of corn pollen collected from Apis mellifera hives compared to floral corn pollen at Nan, Thailand

Fraction

Percentage scavenging activity

EC50

r 2

 

10 µg/ml

50 µg/ml

100 µg/ml

400 µg/ml

1000 µg/ml

(µg/ml)

 

CDEb1

3.76 ± 4.27

0.04 ± 2.05

0.31 ± 2.54

0.00

8.29 ± 2.41

>1000

 

CDEb2

3.16 ± 1.47

0.00

0.00

0.00

5.92 ± 1.85

>1000

 

CDEb3

1.43 ± 2.07

0.1 ± 1.58

0.00

0.00

9.05 ± 0.54

>1000

 

CDEb4

0.00

0.00

0.00

14.35 ± 5.91

12.0 ± 0.54

>1000

 

CDEb5

5.15 ± 0.7

14.0 ± 3.04

22.5 ± 1.86

59.8 ± 3.98

65.3 ± 0.8

322 ± 27

1

CDEb6

3.69 ± 3.7

19.4 ± 3.80

37.6 ± 1.33

76.7 ± 1.2

78.4 ± 1.44

196 ± 9.9

1

CDEb7

3.34 ± 1.33

6.19 ± 2.13

9.69 ± 0.8

38.3 ± 4.8

58.5 ± 1.71

762 ± 67

1

CDEb

66.9 ± 0.88

68.8 ± 0.47

70.8 ± 0.73

70.1 ± 0.47

61.8 ± 0.7

7.47 ± 0.12

1

Ascorbic acid

58.4 ± 1.7

76.9 ± 1.3

76.8 ± 1.05

76.1 ± 0.72

65.2 ± 6.5

8.57 ± 0.24

1

  1. The reference standard, ascorbic acid, is shown for comparison.
  2. EC50 values were calculated by linear regression (r 2 represents the correlation coefficient). ND represents no available data. Data are shown as the means ± 1 SD and are derived from 3 independent repeats.