Figure 3
From: Citrus aurantium flavonoids inhibit adipogenesis through the Akt signaling pathway in 3T3-L1 cells
Effect of CAF on phosophorylation of Akt and GSK3β during 3T3-L1 differentiation. 3T3-L1 preadipocytes were differentiated in the absence or in the presence of CAF for 6 days. (A) Immunoblotting analysis for wild type Akt and pAkt (Ser 473) was described under Materials and Methods. These experiments were conducted as independent experiments in triplicate. Data represent the mean ± SD. (*) p < 0.05. (B) Immunoblotting analysis for wild type GSK3β and pGSK3β (Ser-9) was described under Materials and Methods. These experiments were performed as independent experiments in triplicate. Data represent the mean ± SD. (*) p < 0.05.