Figure 2

Effect of CAF on the expression of adipogenesis-related genes in 3T3-L1 adipocytes. 3T3-L1 cells were differentiated in the absence or presence of CAF for 4 or 6 days. (A) CAF inhibited the expression of adipocyte-specific transcription factors, C/EBPβ and PPARγ mRNA during adipocyte differentiation. The differentiation of 3T3-L1 preadipocytes was induced by DMII medium in the absence or presence of 10 and 50 μg/ml CAF. Total RNA was isolated from 3T3-L1 adipocytes at day 4 and day 6 after induction of differentiation. The expression of C/EBPβ and PPARγ was examined by RT-PCR. (B) The relative expression of adipocyte-specific genes after treating CAF for 4 or 6 days. All gene expressions were normalized using β-actin as a reference gene. The data shown are representative of three independent experiments. (*) p < 0.05, (**) P < 0.01 versus the control (DMII) group at each gene expression. (C) CAF inhibited the expression of adipogenesis-related genes in 3T3-L1 adipocytes. Total cell lysates were isolated from 3T3-L1 adipocytes at day 4 and day 6 after induction of differentiation. Immunoblotting analysis was performed as described in Materials and Methods. (D) The relative expression of adipogenesis-related genes after treating CAF for 4 or 6 days. The data shown are representative of three independent experiments. (*) p < 0.05, (**) P < 0.01, (***) P < 0.001 versus the control (DMII) group at each gene expression.