In vitro effect of CWE on LPS - induced IκBα degradation and MAP kinase activation. Peritoneal macrophages were pretreated with the indicated concentration of CWE for 1 h and then stimulated with LPS for 15 min. Phospho-IKK, IκBα, phospho-IκBα,phospho-JNK, JNK, phospho-p38, p38, phospho-ERK1/2, and ERK1/2 in whole protein extracts were examined by Western blot analysis. Tubulin was used as an internal control. One of the five experiments is shown.