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Figure 4 | BMC Complementary and Alternative Medicine

Figure 4

From: Curcumin induces the apoptosis of human monocytic leukemia THP-1 cells via the activation of JNK/ERK Pathways

Figure 4

JNK and ERK inhibitors reduce the apoptotic effect of curcumin in THP-1 cells. (A) THP-1 cells, which were pre-treated with 50 μM curcumin (CUR) for 2 hours, were incubated with 15 and 30 μM JNK inhibitor (SP600125) for an additional 10 hours. The caspase-3/7 activity of THP-1 cells (1.2 × 104) was determined. Data from 3 independent experiments are presented as mean ± SD. P (*) < 0.05. (B) The same procedure as (A) except that the curcumin-treated cells were incubated with 10 and 20 μM ERK inhibitor (U0126). Data from three independent experiments are presented as mean ± SD. P (*) < 0.05. (C) The same procedure as (A) except that the curcumin-treated cells were incubated with 20 μM ERK inhibitor (CUR + U0126), 30 μM JNK inhibitor (CUR + SP600125) and both (CUR + SP + U). Data from three independent experiments are presented as mean ± SD. P (*) < 0.05. (D) THP-1 cells, which were treated with 50 μM curcumin (CUR) for 2 hours, were incubated with 20 μM ERK inhibitor (U0126) and 30 μM JNK inhibitor (SP600125) for additional 10 hours. Total lysates of the cells were separated and blotted with the indicated antibodies. The data are representative of 3 experiments. (E) THP-1 cells, which were treated with 50 μM curcumin (CUR) for 2 hours, were incubated with 20 μM ERK inhibitor (U0126),30 μM JNK inhibitor (SP600125) or both for additional 10 hours. The cells were stained with propidine iodine (PI), followed by flow cytometry analysis.

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