High-fat-induced attenuation of eNOS protein expression in thoracic aorta and mitigation by DMC and ATV. (A) Representative examples of western blot assay of eNOS (140 kDa) protein are shown including GADPH expression (45 kDa) as a loading control. (B) Statistical bar graph indicates the result of densitometric analysis of the bands as normalized to the quantity of GADPH protein. These ratios were normalized to control. * P < 0.01 vs. control group, # P < 0.05 vs. HF, n = 4.