High-fat-induced loss of vascular reactivity of aortic rings and mitigation by DMC and ATV. (A) Typical tracing and (B) Representative line graph showing increased contraction and relaxation by increasing concentration of PE and ACh. (C) The percent change in relaxation ((tension PE - tension Ach)/tension PE) to 10-4 M ACh after10-5 M PE. (D) Typical tracing and (E) Vascular tension to 60 mM KCl in all groups. Data presented as mean ± S.E.M. and expressed as percentage of baseline tension. * P < 0.01 vs. control group, # P < 0.05 vs. HF, n = 16-18 rings from 6-7 rats.