Mutagenicity and antimutagenicity of (−)-hinokinin a trypanosomicidal compound measured by Salmonella microsome and comet assays

Table 1 DNA migration in the comet assay from V79 cells treated with various doses of HK and/or DXR and their respective controls

Treatments(μM)	Class^*				Score^*	Reduction %;
	0	1	2	3
Control	84.3 ± 9.0	10.0 ± 4.0	2.3 ± 2.0	1.6 ± 2.0	20.3 ± 13.0	-
MeOH	87.0 ± 7.0	11.0 ± 5.5	2.3± 1.5	0	18.0 ± 5.1	-
0.5	81.6 ± 4.9	12.3± 3.5	4.3 ± 3.7	5.0 ± 2.8	26.0 ± 13.2	-
1.0	87.0 ± 5.1	11.0 ± 5.2	1.3 ± 0.5	0	13.0 ± 5.0	-
2.0	83.0 ± 3.7	14.0 ± 2.5	2.0 ± 2.0	0	18.0 ± 5.0	-
32	89.0 ± 2.0	10.0 ± 2.0	0.3 ± 0.5	0.6 ± 1.1	9.6 ± 2.3	-
64	88.0 ± 3.6	11.0 ± 4.0	0.6 ± 1.0	1.0 ± 1.0	13.0 ± 3.4	-
128	89.0 ± 1.5	10.0 ± 1.5	1.0 ± 0.0	0	12.0 ± 1.5	-
DXR	41.0 ± 12.0	36.0 ± 12.7	18.0 ± 2.0	4.0 ± 2.5	86.0 ± 11.5^a	-
DXR + MeOH	46.3 ± 6.8	30.0 ± 6.0	16.3 ± 5.6	6.6 ± 3.0	83.3 ± 14.5^a	-
0.5 + DXR	73.0 ± 7.0	20.0 ± 7.5	4.6 ± 1.5	2.0 ± 2.0	36.0 ± 6.1^a,b	76.0
1.0 + DXR	68.0 ± 2.0	20.6 ± 2.5	8.0 ± 2.0	3.3 ± 0.5	46.0 ± 3.0^a,b	60.8
2.0 + DXR	65.0 ± 6.8	27.0 ± 6.6	4.3 ± 2.3	2.6 ± 1.6	44.3 ± 9.2^a,b	63.4
32 + DXR	35.0 ± 5.0	33.0 ± 3.0	30.0 ± 4.1^b	1.0 ± 1.0	97.0 ± 10.0^a	-
64 + DXR	32.0 ± 6.1	39.0 ± 3.7	26.0 ± 2.0	2.0 ± 2.0	97.0 ± 10.0^a	-
128 + DXR	28.0 ± 6.6	40.0 ± 1.0	29.0 ± 5.2^b	3.0 ± 2.5	106.0 ± 16.0^a	-

HK = (−)-hinokinin; MeOH= methanol (0.1%;); DXR= doxorubicin (0.3 μM).
The different doses (0.5, 1.0, 2.0, 32, 64 and 128 μM) correspond to HK.
^*Values are the means ± standard deviation.
^a Significantly different from control (P<0.05).
^b Significantly different from the DXR group (P<0.05).

ISSN: 2662-7671