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Figure 3 | BMC Complementary and Alternative Medicine

Figure 3

From: Effects of ginsenoside Re on LPS-induced inflammatory mediators in BV2 microglial cells

Figure 3

Ginsenoside-Re attenuates the expression levels of iNOS proteins in LPS-induced BV2 microglial cells. (A) BV2 cells were pretreated with 2 μg/ml of G-Re for 1 h, and then stimulated with 1 μg/ml LPS for 18 h in the presence or absence of 2 μg/ml G-Re. Cells were visualized using immunofluorescence microscopy with antibody against iNOS. Quantification of iNOS expression density by Image J is shown on the right. (n = 5 for each of the four groups.) (bars: 50 μm.) * p < 0.05, ** p < 0.01. (B) Western blots were performed using an antibody against iNOS. The loading control for the cell lysates was determined by re-probing the membranes with α-tubulin antibody. The values shown are the mean ± S.E.M. of data obtained from three independent experiments. * p < 0.05, ** p < 0.01.

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