Figure 1

Effects of ginsenoside-Re on viability in LPS-induced BV2 microglial cells. (A) G-Re inhibits LPS-induced cell death in BV2 microglial cells. BV2 microglial cells were treated with indicated doses (0, 0.05, 0.1, 0.5 and 1 μg/ml) of LPS for 24 h, and cell viability was measured by an MTT assay. The values shown are the mean ± S.E.M. of data obtained from three independent experiments. n=15, * p < 0.05, ** p < 0.01. (B) Effects of G-Re on LPS-induced microglial cell death. BV2 cells were pretreated for 24 h with 2 μg/ml G-Re, and then stimulated with 1 μg/ml LPS for 24 h in the presence or absence of G-Re. The rates of cell survival were measured by a MTT assay at 570 nm. The values shown are the mean ± S.E.M. of data obtained from three independent experiments. (C) BV2 cells were pretreated with 2 μg/ml G-Re for 24 h and then stimulated to 1 μg/ml LPS for 24 h in the presence or absence of 2 μg/ml G-Re. Cells were visualized using immunofluorescence microscopy with antibodies against active caspase-3. The values shown are the mean ± S.E.M. of data obtained from three independent experiments. (bars: 50 μm.) * p < 0.05.