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Effect of Flabellaria paniculata Cav. extracts on gastric ulcer in rats
© Sofidiya et al.; licensee BioMed Central Ltd. 2012
Received: 14 February 2012
Accepted: 24 September 2012
Published: 2 October 2012
The leaves and root of Flabellaria paniculata (Malpighiaceae) are frequently used in the treatment of wounds and ulcers in Nigerian folk medicine. The purpose of this study was to compare the effect of ethanolic extracts from the leaves (FPL) and root (FPR) of F. paniculata on gastric ulcers in rats.
The effect of FPL and FPR (100, 200 and 400 mg/kg) was evaluated in ethanol and indomethacin gastric ulcer models. Control groups for FPL and FPR were orally treated with 3% Tween 20 and distilled water respectively. FPL was further investigated in pylorus ligation model. Misoprostol and cimetidine were used as reference.
FPL significantly (P < 0.05) reduced gastric lesions by 82.22% and 67.32% in ethanol and indomethacin induced ulcer models at 100 mg/kg respectively while FPR (100, 200 and 400 mg/kg) did not exert significant effect in the two models. In pylorus ligation model, FPL exerted a significant preventive antiulcer effect as indicated by reduction in gastric volume at 200 and 400 mg/kg doses. Only 400 mg/kg of the extract exerted a significant reduction in ulcer index when compared with the control group. The oral route LD50 of FPL was estimated to be 4570 mg/kg while that of FPR was 2754 mg/kg. The LD50 in intraperitoneal injection was estimated to be 1202.26 and 1380.38 mg/kg for FPL and FPR respectively. The phytochemical investigation showed that both extracts possess triterpenoids and saponin, while the presence of flavonoid was detected only in FPL.
The results of this study indicated that FPL and not FPR is effective against experimentally induced gastric ulcers. The presence of varied phytochemical constituents probably influenced the pharmacological differences between the two extracts.
Increase consumption of alcohol and non-steroidal anti-inflammatory drugs (NSAID) and inappropriate diets have contributed to the growing ulcer etiopathology around the globe. Thus peptic ulcer is considered a disease of modern times related to increasingly frequent addictions and stressful lifestyle . Besides treatment with synthetic drugs or changes in daily routine, such as nutrition and exercise, other interesting option especially in developing countries, is the use of herbal remedies and preparations.
The genus Flabellaria includes one variable species and is widely distributed in tropical Africa, in the forest, especially along rivers. Flabellaria paniculata Cav. (Malpighiaceae) is a climbing shrub, about 3–15 m high . The lamina of the leaves are broadly elliptic, ovate or rarely lanceolate and the petioles are between 1–2.5 cm long.
The leaves and root of this plant are sold and prescribed as ingredients of herbal preparations in the treatment of wound and ulcers on herbal stalls in Lagos metropolis, Nigeria (informal survey). The leaves are also used in Ghana on wounds and ulcers and as an ecbolic in Côte d'Ivoire . The wound healing and antibacterial property of the leaf has been reported [3, 4]. This study was designed to make a comparative study of the antiulcer activity of leaf (FPL) and root (FPR) extracts of F. paniculata with the aim of establishing which of these extracts is more effective as an antiulcer agent.
Chemicals and drugs
The following drugs and chemicals were used: Absolute ethanol, chloroform, indomethacin (Sigma Chemical Company, St. Louis, MO, USA); Misoprostol (Cytotec® tablet, Pharmaceutical Laboratories, Germany), Cimetidine (Cetilab-400®, Laborate Pharmaceutical, India), Tween 20, Urethane (Aldrich Chemical Co. Ltd, Gillingham Dorset, England).
Adult albino rats and mice of both sexes (100–150 g and 16–30 g respectively) were purchased from the Laboratory Animal Centre, Redeemer University, Ogun State and maintained in the Animal House of College of Medicine, University of Lagos, Nigeria. They were housed in cages and placed on standard pellet feed (Livestock Feed PLC, Ikeja, Lagos, Nigeria) and were given free access to clean water. Each animal was identified by body marks using 1% picric acid solution. They were kept in well ventilated rooms with 12/12 h light/dark conditions and ambient room temperature. Animals were procured two weeks before the experiments to acclimatize with the laboratory environment. The experimental procedures used in this study conform to the United States National Institutes of Health Guidelines for Care and Use of Laboratory Animals in Biomedical Research  and approved by the Committee on Ethics in Animal Experimentation of College of Medicine, University of Lagos, Lagos, Nigeria.
The plant materials were collected from Ilaro, Egbado in Ogun State, Nigeria, in July 2010. The plant samples were authenticated by Mr T. K. Odewo of the herbarium unit of Botany Department, University of Lagos. Voucher specimen (LUH 2778) was deposited in the herbaria of the Department of Botany and Department of Pharmacognosy, University of Lagos.
Preparation of extracts
The leaves were air dried while the root was cut into bits and dried in the oven at 45°C. The dried samples were ground into uniform powder using Christy and Norris 8’ Lab Milling Machine (serial No. 50158). The powdered samples (500 g) were macerated with absolute ethanol (1.5 L) for 48 hours at room temperature with constant shaking. Then, the resulting extracts were filtered over Whatman No. 1 paper and the filtrate evaporated to dryness under vacuum on a rotary evaporator (Heidolph-Rotacool, Germany) at 38°C.
Preliminary phytochemical screening and thin layer chromatography (TLC) were used to evaluate the chemical composition of FPL and FPR crude extracts . FPL was eluted with hexane: ethyl acetate (17:3) while FPR was eluted with butanol: acetic acid: water (4:1:5) and revealed with reagents specific chromophores, dragendorff for alkaloid; anisaldehyde for terpenoids and ferric chloride for phenolic compounds.
Acute toxicity tests
For the assessment of acute toxicity, male and female mice were divided into groups of 5 animals each. FPL and FPR extracts were given p.o. at doses of 50 to 5,000 mg/kg and i.p at doses of 50 to 2000 mg/kg. The control groups received 3% Tween 20 and distilled water (5 ml/kg, p.o.) respectively. The mortality within 24 h period was determined and the LD50 estimated by the log dose–probit analysis method  using GraphPad Prism software.
Ethanol, indomethacin and pylorus ligation were used as ulcerogenic agents in this study. The antiulcer potential of the extracts were tested at three doses (100, 200 and 400 mg/kg). Dissolution of the dried extracts in a common vehicle was a challenge; therefore, two negative controls were used; distilled water (5 ml/kg) for FPR extract treated group and 3% Tween 20 for FPL extract treated group. Misoprostol and cimetidine (100 mg/kg) were used as reference drugs. All drugs and extracts were administered orally.
Ethanol induced gastric ulcer
Indomethacin - induced model
The experiment was carried out according to the method of Ezike et al.  with a few modifications. Forty five fasted animals were used in nine groups of five animals each. Groups 1–6 (FPL and FPR treated group) received 100, 200 and 400 mg/kg of the extracts (p.o), group 7 and 8 received 3% Tween 20 and distilled water, while misoprotol (100 mg/kg) was administered orally for the ninth group. After one hour, all animals received indomethacin 40 mg/kg orally. The rats were sacrificed with ether anesthesia after six hours of drug treatment. The stomachs were isolated, washed gently with normal saline and cut open along the greater curvature. Mucosa lesions were then scored as previously mentioned.
Pylorus ligation (PL)-induced ulcers
Drugs were administered for a period of 5 days by oral gavage and the rats were kept for 18 h fasting . Animals were anaesthetized using urethane (5 ml/kg, i.p.), the abdomen was opened and pylorus ligation was done without causing any damage to its blood supply. The animals were deprived of water during the post-operative period . After 6 h, stomachs were dissected out and cut open along the greater curvature and ulcers were scored in the glandular portion of the stomach. The volume of gastric juice (ml) and pH values were determined. The total acid secretion in the gastric juice supernatant was determined by titration to pH 7.0, using a 0.01 N NaOH solution, and phenolphthalein as indicator.
The results were analyzed using GraphPad Prism version 5.0 software and Student’s t-test was employed for comparing the means between groups. The level of significance was set at p < 0.05.
Preliminary analysis of the chemical composition of FPL and FPR extracts revealed the presence of terpenoids, tannins and saponins while alkaloid was not detected in both extracts. Flavonoid was detected only in the leaf, while anthraquinone was present in the root and not in the leaf.
Acute toxicity test
At higher doses, most of the animals showed increased respiratory rate, hypoactivity, loss of appetite, and dyspnea before death. The oral acute toxicity study produced LD50 values of 4570 and 2754 mg/kg p.o. for FPL and FPR respectively at 24 h. In respect of the i.p. route, no death was recorded between 50 and 100 mg/kg while mortality was 100% at the highest dose of 2000 mg/kg for both FPL and FPR. The LD50 was estimated to be 1202.26 and 1380.38 mg/kg i.p. for FPL and FPR respectively.
Effect on acute gastric mucosal lesions induced by ethanol
Effect of F. paniculata leaf (FPL) and root (FPR) extracts on ethanol-induced gastric ulcers
Control (3% Tween 20)
3.00 ± 0.81
0.53 ± 0.37*
1.25 ± 0.63*
1.73 ± 1.24
4.8 ± 0.73
3.67 ± 0.67
3.8 ± 0.62
3.6 ± 0.81
0.3 ± 0.68 *
FPL (100, 200 and 400 mg/kg) inhibited ulcer formation by 82, 58 and 42% respectively, with the 100 mg/kg dose showing the highest inhibition which is statistically (p < 0.05) different from control (Table 1). FPR (100, 200 and 400 mg/kg) produced a weaker inhibition of gastric lesion with % inhibition of 23, 20 and 25 respectively. The inhibition among these dose groups was not statistically significant (p > 0.05). Misoprostol, the positive control, also offered significant protection (93% inhibition) against ethanol induced gastric lesions and the effect was higher than that of FPL and FPR.
Effect on acute gastric mucosal lesions induced by indomethacin
Effect of F. paniculata leaf (FPL) and root (FPR) extracts on indomethacin induced gastric ulcer
Control (3% Tween 20)
5.1 ± 0.56
1.67 ± 1.08*
2.5 ± 1.14
2.33 ± 0.49*
Control (distilled water)
2.92 ± 0.71
2.08 ± 0.96
4.5 ± 0.82
3.08 ± 0.95
0.2 ± 0.48 *
Effect of FPL extract on pylorus ligation induced gastric ulcers
The data on antiulcerogenic activity of both FPL and FPR in ethanol and indomethacin induced models suggest that FPR failed to reduce the gastric lesions induced by these models; therefore, FPR was discontinued with.
Effect of FPL extract on pylorus ligation induced gastric ulcers
Gastric juice (ml)
Gastric acidity (mEq/l)
Control (3% Tween 20)
1.81 ± 0.5
0.95 ± 0.11
2.89 ± 0.28
11.04 ± 1.76
1.19 ± 0.26
0.72 ± 0.21
2.61 ± 0.41
7.17 ± 0.61
0.69 ± 0.17
0.50 ± 0.13*
2.64 ± 0.19
7.30 ± 0.75
0.37 ± 0.25*
0.22 ± 0.05*
3.10 ± 0.21
6.30 ± 1.41
0.62 ± 0.42
0.86 ± 0.18
3.22 ± 0.33
9.6 ± 0.71
Peptic ulcer is a very prevalent gastrointestinal disorder, characterized by disruption of the mucosal integrity attributed to various aggressive factors (acid, pepsin, stress, Helicobacter pylori, and NSAIDs) and defensive factors (mucus, bicarbonate, blood flow and prostaglandins) . Different therapeutic agents including plant extracts are used to inhibit the gastric acid secretion or to boost the mucosal defense mechanism by increasing mucus production, stabilizing the surface epithelial cells or interfering with the PGs synthesis . This study compares the antiulcer activity of ethanolic extracts of FPL and FPR in ethanol and indomethacin models. Ethanol induced model is used to screen drugs for cytoprotection while indomethacin-induced ulcer model shows both cytoprotection and gastric acid secretion . Furthermore, the effect of FPL in pylorus ligation model was evaluated. This study is the first documentation on the antiulcer activity of this plant.
Ethanol is considered one of the agents that induce more intense gastric ulcers because it promotes serious disturbances in the gastric mucosa . Ethanol induces ulcers by the reduction of gastric mucosal blood flow and mucus production in the gastric lumen, a decrease in endogenous glutathione and prostaglandin levels and an increase of ischemia, gastric vascular permeability, acid ‘back diffusion’, histamine release, efflux of sodium and potassium, influx of calcium, generation of free radicals and production of leukotrienes . In this model, FPL reduced the development of ethanol induced gastric ulcers, with activity highest at a dose of 100 mg/kg. The inhibition was 82%, while misoprostol showed 93% ulcer inhibition when compared to control. This observation indicates gastric cytoprotective effect of FPL. FPR on the other hand failed to inhibit ethanol induced gastric ulcers in rats.
Non-steroidal anti-inflammatory drugs like indomethacin are commonly used pharmacological agents. They induce gastric lesions due to the reduction of endogenous prostaglandin synthesis which is known to be cytoprotective in the gastric mucosa . Compared to the control, administration of FPL inhibited ulcerative lesion, with the highest inhibition recorded for 100 mg/kg dose while FPR extract did not show any significant activity in this model. This observation shows that FPL extract may have protected the gastric mucosa by enhancing prostaglandin synthesis and confirmed the cytoprotective effect of FPL extract.
Considering the data obtained from ethanol and indomethacin ulcer models, the observation that the response of the extracts decreased with increased dose is consistent with findings of studies on some other plants as reported by other authors [19–22]. Such a phenomenon of less response at higher dose is not uncommon with indigenous plants and this observation could be due to ‘therapeutic windows’ effect as suggested by Tripathi  and Zakaria et al. . In other words, it could be that, the effect of the extract has reached its maximum level at a dose of 100 mg/kg, the lowest dose, used in this study.
Pylorus ligation is an important procedure that shows the possible changes of the parameters for gastric content e.g. volume of gastric juice, total acidity and pH . Ulcers caused by pyloric ligation are due to increased accumulation of gastric acid and pepsin, leading to the autodigesion of gastric mucosa . Inhibition of gastric acidity is one of the important protective factors, since overwhelming of the mucosal defense mechanisms by acid level leads to ulcer formation . In this model, only 400 mg/kg of the extract exerted a significant reduction in ulcer index when compared with the control group. FPL exerted a significant preventive antiulcer effect in the pylorus-ligated model as indicated by reduction in gastric volume at 200 and 400 mg/kg doses. These results suggest that the extract interfered with digestive effect of accumulated gastric juice. Gastric acid secretion is stimulated by histamine release from enterochromaffin-like cells in the oxyntic glands; gastrin, released from G cells in the pyloric gastric glands and by acetylcholine, released from postganglionic enteric neurons . The reduction of gastric acidity and gastric secretory volume could be attributed to antihistamine effect, since antihistamine drugs like cimetidine blocks H2 receptors in the stomach thereby reducing the acidity of the gastric juice .
The oral route LD50 of FPL was estimated to be 4570 mg/kg while that of FPR was 2754 mg/kg. The LD50 in intraperitoneal injection was estimated to be 1202.26 and 1380.38 mg/kg for FPL and FPR respectively. Based on the toxicity rating chart  and Hodge and Stemer scale ; the extracts could be classified as being slightly toxic by oral treatment and intraperitoneal injection. Although acute toxicity is a short term study and it does not document any gross organ toxicity, hence, there is the need to carry out a long term toxicity study of the extracts.
The presence of terpenoids, tannins and saponins was detected in FPL and FPR extracts while flavonoids were only present in FPL. Barros et al.  reported that phenolic compounds have an antiulcerogenic effect related to cytoprotective activity. Moreover, flavonoids such as quercetin have been reported to prevent gastric mucosal lesions in various experimental models by increasing the amount of neutral glycoproteins [32, 33]. Flavonoids have been reported to protect the gastric mucosa from damage by increasing the mucosal prostaglandin content and by inhibiting histamine secretion from mast cells by inhibition of histidine decarboxylase . Saponins and triterpenoids have been reported to have antiulcer activity in several experimental models by the formation of protective mucus on the gastric mucosa and also protect the mucosa from acid effects by selectively inhibiting prostaglandin [35, 36]. Also, tannins have been shown to precipitate microproteins at the site of ulcers thereby forming an impervious protective pellicle over the lining to prevent adsorption of toxic substance thereby preventing the attack of proteolytic enzyme. These plant constituents present in FPL might be responsible for the observed activity.
The present study indicated that FPL was more effective against experimentally induced gastric ulcer models. This may justify its use and validated the inclusion of the plant in folk preparations for gastrointestinal remedies in the South Western part of Nigeria. These results provide basis for further studies on FPL. Detailed histological assessment will be part of the follow-up study on FPL, including the bioactivity-guided fractionation to determine the bioactive principle(s) in the plant.
The authors wish to thank Prof. Adeyemi Egunyomi who facilitated the collection of the plant. The technical assistance of Mr. Wale Lasore is highly appreciated. This work was supported by grant from University of Lagos (CRC NO. M2010/03).
- Andreo MA, Ballesteros KVR, Hiruma-Lima CA, da Rocha Machado LR, Souza Brito ARM, Vilegas W: Effect of Mouriri pusa extracts on experimentally induced gastric lesions in rodents: Role of endogenous sulfhydryls compounds and nitric oxide in gastro protection. J Ethnopharmacol. 2006, 107: 431-441. 10.1016/j.jep.2006.04.001.View ArticlePubMedGoogle Scholar
- Burkill HM: The useful plants of West Tropical Africa, Volume 3. 1995, Kew: Royal Botanic Gardens, 2Google Scholar
- Abo KA, Olugbuyiro JAO: Phytochemical and antibacterial studies of extracts of Flabellaria paniculata. Afr J Biomed Res. 2004, 7: 35-36.Google Scholar
- Olugbuyiro JAO, Abo KA, Leigh OO: Wound healing effect of Flabellaria paniculata leaf extract. J Ethnopharmacol. 2010, 127: 786-788. 10.1016/j.jep.2009.10.008.View ArticlePubMedGoogle Scholar
- National Research Council: A Guide for the Care and Use of Laboratory Animals. A report of the Institute of Laboratory Animal Resources Committee on Care and Use of Laboratory Animals. National Institutes of Health Publication No. 85-23. 1985, Washington D.C: National Academy PressGoogle Scholar
- Harborne JB: Phytochemical methods: a guide to modern techniques of plant analysis. 1998, London: Chapman and Hall, 3Google Scholar
- Litchfield JT, Wilcoxon F: A simplified method of evaluating dose effect experiment. J Pharmacol Exp Ther. 1949, 96: 99-113.PubMedGoogle Scholar
- Büyükokuroˇglu ME, Taysi S, Polat F, Göçer F: Mechanism of the beneficial effects of dantrolene sodium on the ethanol-induced acute gastric mucosal injury in rats. Pharmacolog Res. 2002, 45: 421-424. 10.1006/phrs.2002.0951.View ArticleGoogle Scholar
- Magistretti MJ, Conti M, Cristoni A: Antiulcer activity of an anthocyanidin from Vaccinum myrtillus Arzneimittle Forschung. Drug Res. 1988, 38: 686-690.Google Scholar
- Ezike AC, Akah PA, Okoli CO, Ezeuchenne NA, Ezeugwu S: Carica papaya (Paw-Paw) unripe fruit may be beneficial in ulcer. J Med Food. 2009, 12 (6): 1268-1273. 10.1089/jmf.2008.0197.View ArticlePubMedGoogle Scholar
- Anbu Jeba Sunilson J, Varatharajan R, Jayaraj P, John T, Jisha J, Promwichit P: Gastro protective and antioxidant activities of the roots of Hibiscus aculeatus Roxb in rats. Int J Pharmacol. 2008, 4 (4): 252-257. 10.3923/ijp.2008.252.257.View ArticleGoogle Scholar
- Shay H, Komarov SA, Fele SS, Meranze D, Gruenstein H, Siplet H: A simple method for uniform production of gastric ulceration in rat. Gastroenterol. 1945, 5: 43-46.Google Scholar
- Bhatnagar M, Sismondi SS, Bhatnagar R: Antiulcer and antioxidant activity of Asparagus racemosus Willd and Withania somnifera Dunal in Rats. Ann N Y Acad Sci. 2005, 1056: 261-278. 10.1196/annals.1352.027.View ArticlePubMedGoogle Scholar
- Alam MS, Alam MA, Ahmad S, Najimi A, Asif M, Jahangir T: Protective effects of Punica granatum in experimentally-induced gastric ulcers. Toxicol Mech Methods. 2010, 20 (9): 572-578. 10.3109/15376516.2010.508079.View ArticlePubMedGoogle Scholar
- Bighetti AE, Antonio MA, Kohn LK, Rehder VLG, Foglio MA, Possenti A, Vilela L, Carvalho JE: Antiulcerogenic activity of a crude hydroalcoholic extract and coumarin isolated from Mikania laevigata Schultz Bip. Phytomed. 2005, 12: 72-77. 10.1016/j.phymed.2003.09.006.View ArticleGoogle Scholar
- Hiruma-Lima CA, Batista LM, Almeida ABA, Magri LP, Santos LC, Vilegas W, Brito ARMS: Antiulcerogenic action of ethanolic extract of the resin from Virola surinamensis Warb. (Myristicaceae). J Ethnopharmacol. 2009, 122: 406-409. 10.1016/j.jep.2008.12.023.View ArticlePubMedGoogle Scholar
- Glavin GB, Szabo S: Experimental gastric mucosal injury, laboratory models reveal mechanism of pathogenesis and new therapeutic strategies. FASEB J. 1992, 6: 825-831.PubMedGoogle Scholar
- Robert A, Nezamis JE, Lancaster C, Hanchar AJ: Cytoprotection by prostaglandins in rats. Prevention of gastric necrosis produced by alcohol, HCl, NaOH, hypertonic NaCl and thermal injury. Gastroenterol. 1979, 77: 433-443.Google Scholar
- Kesari AN, Gupta RK, Watal G: Hypoglycemic effects of Murraya koenigii on normal and alloxan diabetic rabbits. J Ethnopharmacol. 2005, 97: 247-251. 10.1016/j.jep.2004.11.006.View ArticlePubMedGoogle Scholar
- Singh SK, Kesari AN, Gupta RK, Jaiswal D, Watal G: Assessment of antidiabetic potential of Cynodon dactylon extract in Streptozotocin Diabetic Rats. J Ethnopharmacol. 2007, 114: 174-179. 10.1016/j.jep.2007.07.039.View ArticlePubMedGoogle Scholar
- Rai PK, Jaiswal D, Diwakar S, Watal G: Antihyperglycemic profile of Trichosanthes dioica seeds in experimental models. Pharm Biol. 2008, 46: 360-365. 10.1080/13880200801888011.View ArticleGoogle Scholar
- Singh SK, Mehta S, Jaiswal D, Rai P, Watal G: Antidiabetic effect of Ficus bengalensis aerial roots in experimental animals. J Ethnopharmacol. 2009, 123: 110-114. 10.1016/j.jep.2009.02.017.View ArticlePubMedGoogle Scholar
- Tripathi KD: Essentials of Medical Pharmacology. 2001, New Delhi: Jaypee Brothers Medical Publishers, 52-53.Google Scholar
- Zakaria ZA, Abdul Hisam EE, Rofiee MS, Norhafizah M, Somchit MN, Teh LK, Salleh MZ: In vivo antiulcer activity of the aqueous extract of Bauhinia purpurea leaf. J Ethnopharmacol. 2011, 137: 1047-1054. 10.1016/j.jep.2011.07.038.View ArticlePubMedGoogle Scholar
- Lakshmi V, Singh N, Shrivastva S, Mishra SK, Dharmani P, Mishra V, Palit G: Gedunin and photogedunin of Xylocarpus granatum show significant anti-secretory effects and protect the gastric mucosa of peptic ulcer in rats. Phytomed. 2010, 17: 569-574. 10.1016/j.phymed.2009.10.016.View ArticleGoogle Scholar
- Eswaran MB, Surendran S, Vijayakumar M, Ojha SK, Rawat AKS, Rao CV: Gastroprotective activity of Cinnamomum tamala leaves on experimental gastric ulcers in rats. J Ethnopharmacol. 2010, 128: 537-540. 10.1016/j.jep.2010.01.036.View ArticlePubMedGoogle Scholar
- Arawwawala LDAM, Thabrew MI, Arambewela LSR: Gastroprotective activity of Trichosanthes cucumerina in rats. J Ethnopharmacol. 2010, 127: 750-754. 10.1016/j.jep.2009.11.026.View ArticlePubMedGoogle Scholar
- Biondo TMA, Tanae MM, Coletta ED, Lima-Landman MTR, Lapa AJ, Souccar C: Antisecretory actions of Baccharis trimera (Less.) DC aqueous extract and isolated compounds: Analysis of underlying mechanisms. J Ethnopharmacol. 2011, 136: 368-373. 10.1016/j.jep.2011.04.065.View ArticlePubMedGoogle Scholar
- Loomis TA, Hayes AW: Loomis’s Essentials of Toxicology. 1996, California: Academic, 4Google Scholar
- CCOHS: What is an LD50 and LC50, Canada’s National Occupational Health and Safety Resource: Canadian Centre for Occupational Health and Safety. 2005, [http://www.ccohs.ca/oshanswers/chemicals/ld50.html]Google Scholar
- Barros MP, Lemos M, Maistro EL, Leite MF, Sousa JPB, Bastos JK, Andrade SF: Evaluation of antiulcer activity of the main phenolic acids found in Brazilian green propolis. J Ethnopharmacol. 2008, 120: 372-377. 10.1016/j.jep.2008.09.015.View ArticlePubMedGoogle Scholar
- Di Carlo G, Mascolo N, Izzo AA: Flavonoids: old and new aspects of a class of natural therapeutic drugs. Life Sci. 1999, 65: 337-353. 10.1016/S0024-3205(99)00120-4.View ArticlePubMedGoogle Scholar
- Zayachkivska OS, Konturek SJ, Drozdowicz D, Konturek PC, Brzozowski T: Gastroprotective effects of flavonoids in plant extracts. J Physiol Pharmacol. 2005, 56: 219-231.PubMedGoogle Scholar
- Borelli F, Izzo AA: The plant kingdom as a source of antiulcer remedies. Phytotherapy Res. 2000, 14: 581-591. 10.1002/1099-1573(200012)14:8<581::AID-PTR776>3.0.CO;2-S.View ArticleGoogle Scholar
- Agwu CN, Okunji CO: Gastrointestinal studies of Pyrenacantha staudii leaf extracts. J Ethnopharmacol. 1986, 15: 45-55. 10.1016/0378-8741(86)90103-0.View ArticleGoogle Scholar
- Lewis DA, Hanson PJ: Anti-ulcer drugs of plant origin. Progress in medicinal chemistry 28. Edited by: Ellis GP. 1991, West GB Amsterdam: Elsevier Science Publishers, 201-231.Google Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6882/12/168/prepub
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