Figure 4
AST suppresses COX-2 and VEGF induction under normoxia and hypoxia in colon cancer cells. (A) Effects of AST alone {A} (60 μg/ml) or in combined treatment with indomethacin {A + I} on the level of COX-2 in HT-29 cells under normoxia. (B) Combined treatment of AST (80 μg/ml) and indomethacin (100 μM) suppressed the induced COX-2 and VEGF level under CoCl2-mimicked hypoxic condition in HCT 116 cells. Data represents the mean of at least three independent experiments. β-actin was used as internal control in immunoblot assay. Arbitrary data are expressed as mean ± S.E.M., with statistical significance * p < 0.05, ** p < 0.01 when compared with the untreated group at 0 h or control group; # p < 0.05, ## p < 0.01 when compared with CoCl2 hypoxia group; + p < 0.05 when compared with indomethacin (A) or CoCl2 + indomethacin (B) group.