Figure 5

Water soluble extract of P. linteus (WA) inhibits the expression of pathogenic cytokines and chemokines in ear. After final treatment, mice of each group were sacrificed and ears were removed. (A, B) Total ear cells were stimulated with PMA (0.5 μg/ml) and ionomycin (1 μM) for 4 hrs, then mRNA expression of chemokines (A) and cytokines (B) were determined by quantitative RT-PCR. (C) CD4+ T cells isolated from ear total cells were stimulated and cytokine levels were measured by quantitative RT-PCR. The mRNA expression levels in each group were normalized with L32 (house- keeping gene) and then fold induction of each target gene were compared to Cont (PBS) group. Error bars indicated S.D. One (*), Two (**) and three asterisk (***) indicates P < 0.05, P < 0.005, P < 0.001, respectively. Data are representative of three independent experiments.