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Table 3 Effect of D. gnidium extracts on the genotoxicity induced by nitrofurantoin (5 μg/assay)

From: Antioxidant, genotoxic and antigenotoxic activities of daphne gnidium leaf extracts

Extracts Doses (μg/assay) β-gal (UE) PA (UE) IF Inhibition of genotoxicity (%)
Nitrofurantoin 5 42,18 ± 0,092 11,19 ± 0,0021 23,56 -
NC 0 3,07 ± 0,024 18,58 ± 0,007 1 -
Petroleum ether extract 50 26,21 ± 0,011 9,82 ± 0,019 16,68 30,50*
  250 26,65 ± 0,041 9,61 ± 0,011 17,31 27,70*
  500 27,5 ± 0,019 9,87 ± 0,007 17,37 27,43*
Chloroform extract 50 14,31 ± 0 7,05 ± 0 12,29 49,90**
  250 16,18 ± 0,0169 10,29 ± 0 9,53 62,19**
  500 4,66 ± 0,047 4,45 ± 0 6,35 76,28**
Ethyl acetate extract 5 8,19 ± 0,019 5,21 ± 0 9,53 62,18**
  15 7,18 ± 0,0183 5,29 ± 0,0028 8,23 67,95**
  30 7,93 ± 0,0127 5,68 ± 0,007 8,46 66,93**
Methanol extract 50 14,67 ± 0,001 9,48 ± 0,08 9,38 62,85**
  100 15,98 ± 0,08 10,55 ± 0,04 9,18 63,74**
  200 15,42 ± 0,004 12,464 ± 0,042 7,50 71,18**
TOF extract 5 19,73 ± 0,014 8,41 ± 0,009 14,66 39,45*
  15 17,68 ± 0,029 7,04 ± 0,007 15,68 34,92*
  30 14,76 ± 0,009 7,17 ± 0,004 12,87 47,38*
  1. β-gal: units of β-galactosidase; AP: units of phosphatase alkaline; IF: induction factor.
  2. Positive control of genotoxicity (nitrofurantoin); NC: negative control (non-treated cells).
  3. Results were expressed as percentage of inhibition of genotoxicity compared to the positif control (Nitrofurantoin). Values represent the mean ± SD of three separate experiments. The statistical significance of results was evaluated by the Student’s t-test. *P < 0.05, **P < 0.01 means significant difference between positif control and treated sample.